Analytical Data
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基因名
NDST3
- Application
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别名
NDST3; HSST3; UNQ2544/PRO4998Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 3; EC 2.8.2.8; Glucosaminyl N-deacetylase/N-sulfotransferase 3; NDST-3; hNDST-3; N-heparan sulfate sulfotransferase 3; N-HSST 3) [Includes: Heparan sulfate N-deacetylase 3; EC 3.-.-.-); Heparan sulfate N-sulfotransferase 3; EC 2.8.2.-)]
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O95803
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表达区间
162-259 aa
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氨基酸序列
IGFHKTSEKSVQSFQLKGFPFSIYGNLAVKDCCINPHSPLIRVTKSSKLEKGSLPGTDWTVFQINHSAYQPVIFAKVKTPENLSPSISKGAFYATIIH
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分子量
36.52 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NDST3 (N-deacetylase/N-sulfotransferase 3) is an enzyme belonging to the NDST family, which plays a crucial role in the modification of heparan sulfate, a glycosaminoglycan involved in various biological processes. The heparan sulfate modification is essential for cell signaling, development, and tissue homeostasis. NDST3 specifically catalyzes the addition of sulfate groups to the N-acetylated glucosamine residues in heparan sulfate chains, influencing their biological activity and interactions with various signaling molecules. Abnormalities in NDST3 function have been linked to several diseases, including cancer and neurodevelopmental disorders, highlighting its potential as a therapeutic target. Researchers have increasingly focused on understanding the biochemical properties, structural characteristics, and substrate specificity of NDST3 to elucidate its role in cellular processes and disease mechanisms. Recent advances in recombinant protein technology have enabled the production of functional NDST3 for in vitro studies, facilitating investigations into its enzymatic activity and interactions with heparan sulfate precursors. This research not only enhances the understanding of NDST3's biological functions but also paves the way for the development of novel therapeutic strategies aimed at modulating heparan sulfate modifications in pathological conditions.
