Analytical Data
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Gene name
Angiopoietin-1
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简介
The Angiopoietin-1 Protein is a secreted glycoprotein that is a key ligand in the vascular tyrosine kinase signaling pathway. ANGPT1 stimulates angiogenesis by activating PTK2/FAK and downstream kinase MAPK1/ERK2 and MAPK3/ERK1 signaling pathways. ANGPT1 affects the proliferation, migration and differentiation of skeletal muscle cells. Angiopoietin-1 Protein, Cynomolgus (HEK293, Fc) is the recombinant cynomolgus-derived Angiopoietin-1 protein, expressed by HEK293 , with N-hFc labeled tag.
- Application
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Biological Activity
Determined by its ability to induce adhesion of human umbilical vein endothelial cells (HUVEC).The ED50 for this effect is 252.3 ng/mL. Determined by its ability to induce adhesion of human umbilical vein endothelial cells (HUVEC).The ED50 for this effect is 252.3 ng/mL, corresponding to a specific activity is 3963.535 units/mg.
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Alternative Names
AGP1; AGPT; ANG-1; Angiopoietin-1; ANGPT1
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Species
Cynomolgus
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Source
HEK293
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Tag
N-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
XP_005563980
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Expression Region
R277-F498
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Protein Length
Partial
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Molecular Weight
54.1 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Angiopoietin-1 (Ang-1) is a crucial protein that plays a significant role in angiogenesis, the process of new blood vessel formation, which is essential for maintaining vascular stability and regulating inflammation. It is part of the Angiopoietin/Tie signaling pathway, where Ang-1 binds to its receptor Tie-2, facilitating vascular maturation and stabilization. Research has highlighted Ang-1's potential in therapeutic applications, particularly in conditions such as cardiovascular diseases, cancer, and wound healing, where angiogenesis is often disrupted. Recombinant Angiopoietin-1 proteins are being studied for their ability to enhance endothelial cell survival, proliferation, and migration, ultimately promoting healthy blood vessel formation. Additionally, Ang-1's anti-inflammatory properties are of interest, as they could mitigate tissue injury and promote repair mechanisms. As scientists explore the molecular mechanisms underlying Ang-1's functions, recombinant protein technology offers opportunities for developing novel treatments that harness the protective effects of Ang-1, making it a promising candidate in regenerative medicine and vascular therapy.
