Analytical Data
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基因名
IL-12
- Application
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别名
IL-12；NKSF1；Interleukin-12 subunit alpha
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P43432
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表达区间
23-335aa
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氨基酸序列
MWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIGSG KTLTITVKEFLDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKN KTFLKCEAPNYSGRFTCSWLVQRNMDLKFNIKSSSSSPDSRAVTCGMASL SAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELALEARQQNKYENYS TSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFV RIQRKKEKMKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSS CSKWACVPCRVRS
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分子量
36 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Recombinant interleukin-12 (IL-12) is a crucial cytokine involved in the regulation of immune responses, particularly in driving Th1 cell differentiation and enhancing cytotoxic T cell activity. Initially discovered for its ability to stimulate T and natural killer (NK) cells, IL-12 has since garnered significant attention in immunotherapy and vaccine development, especially for cancer and infectious diseases. The molecular structure of IL-12, which consists of p35 and p40 subunits, allows for its use in recombinant protein formats that can boost immune responses. Research over the past few decades has demonstrated that IL-12 can potentiate anti-tumor immunity and improve outcomes in various preclinical and clinical settings. However, its clinical application has been limited by potential toxicity and the need for careful dosage management. Recent studies focus on optimizing delivery methods, such as gene therapy and targeted delivery mechanisms, to enhance its therapeutic efficacy while minimizing adverse effects. The continued exploration of recombinant IL-12 is pivotal in harnessing its immunomodulatory properties, aiming to advance therapeutic strategies and pave the way for innovative treatments in oncology and beyond.
