Analytical Data
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Gene name
AKR1C2
- Application
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Alternative Names
AKR1C2；DDH2；Aldo-keto reductase family 1 member C2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P52895
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Expression Region
1-323aa
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AA Sequence
MDSKYQCVKLNDGHFMPVLGFGTYAPAEVPKSKALEAVKLAIEAGFHHID SAHVYNNEEQ VGLAIRSKIADGSVKREDIFYTSKLWSNSHRPELVRPALERSLKNLQLDY VDLYLIHFPV SVKPGEEVIPKDENGKILFDTVDLCATWEAMEKCKDAGLAKSIGVSNFNH RLLEMILNKP GLKYKPVCNQVECHPYFNQRKLLDFCKSKDIVLVAYSALGSHREEPWVDP NSPVLLEDPV LCALAKKHKRTPALIA
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Molecular Weight
29 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
AKR1C2, a member of the aldo-keto reductase (AKR) superfamily, plays a significant role in the metabolism of steroid hormones, prostaglandins, and various xenobiotics. Research into AKR1C2 has garnered attention due to its implications in several physiological and pathological processes, including hormone regulation, cancer progression, and metabolic disorders. The enzyme is involved in the reduction of androstenedione to testosterone, highlighting its importance in androgen metabolism, which can impact prostate cancer and other hormone-related diseases. Furthermore, AKR1C2 has been implicated in the detoxification processes of reactive carbonyl species, underscoring its potential role in cellular protection against oxidative stress. The recombinant expression of AKR1C2 has enabled more detailed studies on its enzymatic properties, substrate specificity, and potential as a therapeutic target. Investigating the structure-function relationship of AKR1C2 through techniques such as crystallography and mutagenesis has provided insights into its molecular mechanisms and interaction with various ligands. Given the increasing awareness of the enzyme's role in drug metabolism and disease, further research into AKR1C2 may pave the way for novel therapeutic strategies and biomarkers for disease prognosis. This highlights the necessity of continued exploration into its functional characteristics and regulatory mechanisms, which could ultimately facilitate advancements in personalized medicine and treatment modalities for conditions influenced by steroid hormone metabolism.
