Analytical Data
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基因名
PTCH
- Application
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别名
Protein patched homolog 1. PTC. PTC1
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q13635
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表达区间
1-183 aa
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氨基酸序列
MGKATGRKAPLWLRAKFQRLLFKLGCYIQKNCGKFLVVGLLIFGAFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLL
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分子量
47.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PTCH (Patched) is a key receptor in the Hedgehog signaling pathway, which plays a critical role in various developmental processes and tissue homeostasis. Mutations in the PTCH gene are associated with several types of cancers, including basal cell carcinoma and medulloblastoma, highlighting its importance as a tumor suppressor. In recent years, the focus on PTCH has increased due to its potential as a therapeutic target in cancer treatment. Researchers have been investigating the structure and function of PTCH and its interactions with Hedgehog ligands, leading to insights into the molecular mechanisms underlying signaling regulation. Recombinant PTCH proteins are being produced to facilitate these studies, allowing for detailed analyses of PTCH's role in signal transduction and its implications in cancer biology. The availability of PTCH recombinant proteins has opened avenues for drug discovery, enabling high-throughput screening of small molecules that can modulate this pathway. Overall, the study of PTCH and its recombinant forms continues to be a pivotal area of research, providing valuable information for the development of innovative cancer therapies based on the modulation of the Hedgehog signaling pathway.
