Analytical Data
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基因名
PUSL1
- Application
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别名
PUSL1; tRNA pseudouridine synthase-like 1; EC 5.4.99.-; tRNA pseudouridylate synthase-like 1; tRNA-uridine isomerase-like 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8N0Z8
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表达区间
1-303 aa
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氨基酸序列
MSSAPASGSV RARYLVYFQY VGTDFNGVAA VRGTQRAVGV QNYLEEAAER LNSVEPVRFT ISSRTDAGVH ALSNAAHLDV QRRSGRPPFP PEVLAEALNT HLRHPAIRVL RAFRVPSDFH ARHAATSRTY LYRLATGCHR RDELPVFERN LCWTLPADCL DMVAMQEAAQ HLLGTHDFSA FQSAGSPVPS PVRTLRRVSV SPGQASPLVT PEESRKLRFW NLEFESQSFL YRQVRRMTAV LVAVGLGALA PAQVKTILES QDPLGKHQTR VAPAHGLFLK SVLYGNLGAA SCTLQGPQFG SHG
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分子量
33.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PUSL1, or Pseudouridine Synthase and Leucine-rich Repeat Protein 1, has emerged as a significant research target due to its role in RNA modification and cellular function. Pseudouridine is a modified nucleoside found in various RNA molecules, and its synthesis is facilitated by enzymes known as pseudouridine synthases. PUSL1 belongs to this family and is thought to play a critical role in post-transcriptional RNA processing, influencing mRNA stability, translation efficiency, and the overall dynamics of gene expression. Increased evidence suggests that PUSL1 may be linked to various biological processes, including stress response, cell differentiation, and proliferation. Moreover, alterations in its expression or function have been associated with several diseases, including cancer and neurological disorders. Investigating PUSL1 could provide insights into its mechanistic roles in RNA biology and potential therapeutic targets for diseases resulting from dysregulated RNA modifications. This growing interest underscores the necessity for rigorous studies to elucidate PUSL1’s structure, enzymatic activity, and broader implications in cellular homeostasis and pathology.
