Analytical Data
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基因名
PUS7L
- Application
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别名
PUS7L; Pseudouridylate synthase 7 homolog-like protein; EC 5.4.99.-
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9H0K6
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表达区间
1-701 aa
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氨基酸序列
MEEDTDYRIR FSSLCFFNDH VGFHGTIKSS PSDFIVIEID EQGQLVNKTI DEPIFKISEI QLEPNNFPKK PKLDLQNLSL EDGRNQEVHT LIKYTDGDQN HQSGSEKEDT IVDGTSKCEE KADVLSSFLD EKTHELLNNF ACDVREKWLS KTELIGLPPE FSIGRILDKN QRASLHSAIR QKFPFLVTVG KNSEIVVKPN LEYKELCHLV SEEEAFDFFK YLDAKKENSK FTFKPDTNKD HRKAVHHFVN KKFGNLVETK SFSKMNCSAG NPNVVVTVRF REKAHKRGKR PLSECQEGKV IYTAFTLRKE NLEMFEAIGF LAIKLGVIPS DFSYAGLKDK KAITYQAMVV RKVTPERLKN IEKEIEKKRM NVFNIRSVDD SLRLGQLKGN HFDIVIRNLK KQINDSANLR ERIMEAIENV KKKGFVNYYG PQRFGKGRKV HTDQIGLALL KNEMMKAIKL FLTPEDLDDP VNRAKKYFLQ TEDAKGTLSL MPEFKVRERA LLEALHRFGM TEEGCIQAWF SLPHSMRIFY VHAYTSKIWN EAVSYRLETY GARVVQGDLV CLDEDIDDEN FPNSKIHLVT EEEGSANMYA IHQVVLPVLG YNIQYPKNKV GQWYHDILSR DGLQTCRFKV PTLKLNIPGC YRQILKHPCN LSYQLMEDHD IDVKTKGSHI DETALSLLIS FDLDASCYAT VCLKEIMKHD V
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分子量
80.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PUS7L (Pseudouridine Synthase 7-Like) is a highly conserved enzyme within the pseudouridine synthase family, playing a critical role in RNA metabolism by catalyzing the conversion of specific uridine residues in RNA to pseudouridine. This modification is essential for the structural stability and functional integrity of various RNA molecules, including tRNA, rRNA, and snRNA. Recent studies have highlighted the significance of pseudouridine in regulating gene expression and influencing the cellular response to environmental stresses. Mutations or dysregulation of PUS7L have been associated with various diseases, including cancer and genetic disorders, suggesting that understanding its molecular mechanisms could provide insights into potential therapeutic targets. Moreover, the role of PUS7L in the context of non-coding RNAs and ribonucleoprotein complexes has garnered attention, positioning it as a pivotal player in the epitranscriptomic landscape. As researchers continue to explore the functional implications of PUS7L and its interactions with RNA and other cellular components, this protein may reveal novel insights into post-transcriptional regulation and its impact on cellular homeostasis. Therefore, the investigation of PUS7L offers valuable opportunities to unravel the complexities of RNA modification and its implications in health and disease.
