Analytical Data
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基因名
STIM1
- Application
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别名
STIM1；GOK；Stromal interaction molecule 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q13586-1
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表达区间
1-213aa
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氨基酸序列
MDVCVRLALW LLWGLLLHQG QSLSHSHSEK ATGTSSGANS EESTAAEFCR IDKPLCHSED EKLSFEAVRN IHKLMDDDAN GDVDVEESDE FLREDLNYHD PTVKHSTFHG EDKLISVEDL WKAWKSSEVY NWTVDEVVQW LITYVELPQY EETFRKLQLS GHAMPRLAVT NTTMTGTVLK MTDRSHRQKL QLKALDTVLF GPPLLTRHNH LKD
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分子量
23 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
STIM1, a crucial protein in cellular calcium homeostasis, serves as a sensor for calcium levels in the endoplasmic reticulum (ER). When intracellular calcium levels drop, STIM1 undergoes a conformational change, leading to its translocation to the ER-plasma membrane junctions, where it activates store-operated calcium entry (SOCE) by interacting with Orai1 channels. This mechanism is critical for various physiological processes, including muscle contraction, immune response, and neuronal signaling. Abnormalities in STIM1 function have been linked to several pathologies, such as immunodeficiencies, cardiac diseases, and neurological disorders. Given its fundamental role, the study of STIM1 recombinant proteins has gained significant attention, particularly in understanding its structure-function relationships and the regulatory mechanisms of SOCE. Producing STIM1 as a recombinant protein enables detailed biochemical assays and structural studies, facilitating insights into how STIM1 operates at the molecular level. Additionally, recombinant STIM1 can be used in the development of targeted therapies aimed at modulating calcium signaling in diseases associated with dysregulated STIM1 activity. Therefore, research on STIM1 recombinant proteins not only contributes to the fundamental biology of calcium signaling but also holds promise for therapeutic advancements in related diseases.
