Cat: PA2000-2400

Recombinant Human Mcpt4 Protein,His

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Analytical Data

  • Gene name

    Mcpt4

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    Mcpt4;Mast cell protease 4

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    P21812

  • Expression Region

    21-246aa

  • AA Sequence

    IIGGVESRPHSRPYMAHLEITTERGFTATCGGFLITRQFVMTAAHCSGREITVTLGAHDVSKTESTQQKIKVEKQIVHPKYNFYSNLHDIMLLKLQKKAKETPSVNVIPLPRPSDFIKPGKMCRAAGWGRTGVTEPTSDTLREVKLRIMDKEACKNYWHYDYNLQVCVGSPRKKRSAYKGDSGGPLLCAGVAHGIVSYGRGDAKPPAVFTRISSYVPWINRVIKGE

  • Molecular Weight

    29.1kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

The research on Mcpt4 recombinant protein focuses on its role within the immune system, specifically in the context of mast cell function and allergic reactions. Mcpt4, also known as mouse mast cell protease 4, is a serine protease primarily expressed in mast cells and plays a crucial role in inflammatory responses. Its unique positioning in the immune pathway makes it a candidate for understanding the mechanisms underlying various allergic conditions and mast cell-related diseases. Studies have shown that Mcpt4 is involved in the regulation of cytokine production, influencing both innate and adaptive immune responses. The recombinant form of this protein allows researchers to investigate its biochemical properties, enzymatic activities, and potential interactions with other immune components, providing insights into its functional significance. By exploring the therapeutic potential of Mcpt4, researchers aim to develop targeted strategies for managing allergic disorders and enhancing our understanding of mast cell biology. This research is significant as it could lead to novel interventions for allergic diseases, which affect a substantial portion of the population worldwide.

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