Analytical Data
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Gene name
glpD
- Application
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Alternative Names
glpD；glyD；Aerobic glycerol-3-phosphate dehydrogenase
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P13035
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Expression Region
1-501aa
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AA Sequence
METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSASSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIRIGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVVRKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGMHLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYKGDPKAVKIEESEINYLLNVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDYTLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIEGDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAELKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQRLSLAS
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Molecular Weight
60.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of glpD recombinant protein has gained significant attention due to its crucial role in microbial metabolism and potential applications in biotechnology. GlpD, or glycerol-3-phosphate dehydrogenase, is an enzyme involved in the glycerolipid biosynthesis pathway, facilitating the conversion of glycerol-3-phosphate into dihydroxyacetone phosphate. This enzyme is vital for maintaining cellular energy balance and membrane homeostasis in various organisms, including bacteria and eukaryotes. Understanding the structure and function of glpD can provide insights into the regulatory mechanisms of lipid metabolism, which is essential for the survival and growth of microorganisms in diverse environments. Moreover, recombinant glpD proteins have potential applications in metabolic engineering and synthetic biology, where they can be utilized to optimize lipid production in microbial cell factories. By harnessing the capabilities of glpD, researchers aim to develop more efficient bioprocesses for the production of biofuels, pharmaceuticals, and other valuable biochemicals. Recent advancements in recombinant DNA technology have enabled the successful expression and purification of glpD from various sources, facilitating detailed kinetic and structural studies. These findings pave the way for innovative strategies to manipulate lipid biosynthesis pathways for industrial applications.
