Analytical Data
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基因名
ctfA
- Application
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别名
ctfA；Acetoacetyl-CoA:acetate/butyrate CoA transferase alpha subunit
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P33752
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表达区间
1-218aa
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氨基酸序列
MNSKIIRFENLRSFFKDGMTIMIGGFLNCGTPTKLIDFLVNLNIKNLTIISNDTCYPNTGIGKLISNNQVKKLIASYIGSNPDTGKKLFNNELEVELSPQGTLVERIRAGGSGLGGVLTKTGLGTLIEKGKKKISINGTEYLLELPLTADVALIKGSIVDEAGNTFYKGTTKNFNPYMAMAAKTVIVEAENLVSCEKLEKEKAMTPGVLINYIVKEPA
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分子量
23.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The CtfA protein, encoded by the ctfA gene, plays a crucial role in various biological processes, particularly in bacterial cell division and biofilm formation. Research into CtfA primarily centers around its function as an essential protein in the assembly of the divisome, the complex responsible for bacterial cytokinesis. Deletion or mutation of the ctfA gene often leads to significant defects in cell morphology and division, highlighting its importance in maintaining cellular integrity and normal growth patterns. Beyond its role in cell division, CtfA has also been implicated in the regulation of biofilm formation, a critical factor in bacterial survival and persistence in various environments. Studies have indicated that CtfA interacts with other proteins within the divisome and potentially influences the dynamics of this assembly, thereby impacting the overall process of cell division. The investigation into CtfA not only enhances our understanding of bacterial physiology but also holds potential implications for the development of novel antimicrobial strategies aimed at disrupting bacterial growth and biofilm development. Understanding the structural and functional properties of CtfA could lead to innovative approaches in combating bacterial infections and biofilm-related complications, making it a significant target for future research in microbiology and biotechnology.
