Analytical Data
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Gene name
NIM1
- Application
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Alternative Names
NIM1；NIM1；Serine/threonine-Protein kinase NIM1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8IY84
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Expression Region
1-436aa
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AA Sequence
MTAVYMNGGGLVNPHYARWDRRDSVESGCQTESSKEGEEGQPRQLTPFEKLTQDMSQDEKVVREITLGKRIGFYRIRGEIGSGNFSQVKLGIHSLTKEKVAIKILDKTKLDQKTQRLLSREISSMEKLHHPNIIRLYEVVETLSKLHLVMEYAGGGELFGKISTEGKLSEPESKLIFSQIVSAVKHMHENQIIHRDLKAENVFYTSNTCVKVGDFGFSTVSKKGEMLNTFCGSPPYAAPELFRDEHYIGIYVDIWALGVLLYFMVTGTMPFRAETVAKLKKSILEGTYSVPPHVSEPCHRLIRGVLQQIPTERYGIDCIMNDEWMQGVPYPTPLEPFQLDPKHLSETSTLKEEENEVKSTLEHLGITEEHIRNNQGRDARSSITGVYRIILHRVQRKKALESVPVMMLPDPKERDLKKGSRVYRGIRHTSKFCSIL
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Molecular Weight
65.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NIM1, a protein originally identified in the context of cellular stress responses, has garnered interest in the fields of molecular biology and biotechnology due to its potential implications in various biological processes, including signal transduction and cell cycle regulation. Studies have shown that NIM1 plays a critical role in cellular defense mechanisms against environmental stressors such as oxidative stress and pathogens. Its involvement in modulating gene expression and protein stability further highlights its significance in maintaining cellular homeostasis. The recombinant production of NIM1 allows for detailed characterization of its structural and functional properties, facilitating the exploration of its biochemical pathways and interactions with other cellular components. Moreover, understanding the functional mechanisms of NIM1 can provide insights into its potential therapeutic applications, particularly in designing strategies for mitigating stress-induced cellular damage or in enhancing stress tolerance in plants. Therefore, the research into NIM1 recombinant protein not only contributes to the fundamental knowledge of stress response mechanisms but also opens avenues for biotechnological applications that could enhance crop resilience in changing environmental conditions. As scientists continue to characterize this protein, the implications of its study extend from basic research to practical applications in agriculture and medicine.
