Analytical Data
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基因名
KIR2DL5/CD158f
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简介
KIR2DL5/CD158f, present on NK cells, acts as a receptor for HLA-C alleles. Its inhibitory function finely tunes NK cell activity, preventing cell lysis by interacting with specific HLA-C molecules. This dynamic engagement contributes to the nuanced balance of inhibitory signals in the immune system, regulating NK cell responses to potential targets. KIR2DL5/CD158f Protein, Human (HEK293, Fc) is the recombinant human-derived KIR2DL5/CD158f protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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生物活性
Immobilized Human KIR2DL5 at 2 μg/mL (100 μL/well) can bind Anti- KIR2DL5 Antibody. The ED50 for this effect is 1.558 μg/mL. Immobilized Human KIR2DL5 at 2 μg/mL (100 μL/well) can bind Anti- KIR2DL5 Antibody. The ED50 for this effect is 1.558 μg/mL.
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别名
Killer cell immunoglobulin-like receptor 2DL5A; CD158f1; KIR2DL5A; CD158F
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种属
Human
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表达系统
HEK293
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标签
C-hFc
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q8N109
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表达区间
H22-H240
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氨基酸序列
HEGGQDKPLLSAWPSAVVPRGGHVTLLCRSRLGFTIFSLYKEDGVPVPELYNKIFWKSILMGPVTPAHAGTYRCRGSHPRSPIEWSAPSNPLVIVVTGLFGKPSLSAQPGPTVRTGENVTLSCSSRSSFDMYHLSREGRAHEPRLPAVPSVNGTFQADFPLGPATHGGTYTCFGSLHDSPYEWSDPSDPLLVSVTGNSSSSSSSPTEPSSKTGIRRHLH
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蛋白长度
Partial
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分子量
65 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KIR2DL5, also known as CD158f, is a member of the Killer Immunoglobulin-like Receptor (KIR) family, which plays a significant role in the regulation of immune responses. KIR2DL5 is primarily expressed on the surface of natural killer (NK) cells and has been implicated in the recognition of target cells, influencing the activation and inhibition of NK cell functions. The investigation of KIR2DL5/CD158f is particularly relevant in the context of its potential involvement in immune evasion by tumors and pathogens, as well as its impact on graft-versus-host disease in transplant settings. Understanding the precise molecular mechanisms by which KIR2DL5 interacts with its ligands and modulates NK cell activity is crucial for the development of immunotherapies and novel treatment strategies for various diseases, including cancers and viral infections. Additionally, the generation of recombinant KIR2DL5 proteins is essential for functional studies, enabling researchers to analyze receptor-ligand interactions and the downstream signaling pathways triggered upon activation. The advancement of this research could lead to improved therapeutic approaches that enhance NK cell responses in oncology and transplantation, thereby offering new avenues for patient management and treatment outcomes.
