Analytical Data
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Gene name
Apolipoprotein E/APOE4
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简介
Apolipoprotein E (APOE) plays a key role in lipoprotein-mediated lipid transport and is a core component in the production, transformation, and clearance of plasma lipoproteins. As an amphipathic molecule, APOE binds to various lipoprotein particles, including chylomicrons, chylomicron remnants, VLDL, and IDL, favoring HDL. Apolipoprotein E/APOE Protein, Human (C130R, HEK293, Fc) is the recombinant human-derived Apolipoprotein E/APOE protein, expressed by HEK293 , with N-hFc labeled tag and C130R mutation.
- Application
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Alternative Names
Apolipoprotein E; Apo-E; APOE; apolipo E; APOE4
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Species
Human
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Source
HEK293
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Tag
N-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P02649
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Expression Region
K19-H317, C130R
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Protein Length
Full Length of Mature Protein
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Molecular Weight
63-67 kDa (Due to glycosylation)
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Apolipoprotein E (ApoE) is a crucial protein involved in lipid metabolism and neurodegenerative diseases, particularly Alzheimer’s disease (AD). The ApoE gene exists in three major isoforms: ApoE2, ApoE3, and ApoE4, with ApoE4 being a significant genetic risk factor for late-onset AD. Research indicates that individuals possessing the ApoE4 allele have a higher likelihood of developing AD and an earlier onset of cognitive decline compared to those with other isoforms. The pathogenic mechanisms associated with ApoE4 are not fully understood but are believed to relate to altered lipid transport, neuroinflammation, and amyloid-beta deposition in the brain. The study of recombinant ApoE4 proteins is pivotal for elucidating these mechanisms. By isolating and characterizing recombinant ApoE4, researchers can investigate its structural properties, interaction with lipids and receptors, and its impact on cellular processes. Moreover, understanding the differences between ApoE isoforms can aid in developing targeted therapies and interventions for patients at risk for AD. Exploring recombinant ApoE4 also offers insights into potential biomarker development and novel therapeutic strategies that could mitigate its deleterious effects in neurodegenerative diseases. Overall, the investigation of recombinant ApoE4 proteins contributes significantly to the understanding of ApoE's role in lipid metabolism and its implications for Alzheimer's pathology.
