Recombinant Human LRPAP1 Protein (HEK293)，His

LRPAP1

The LRPAP1 protein serves as a molecular chaperone for LDL receptor-related proteins and regulates ligand binding throughout the secretory pathway. It transiently interacts with the LRP1/α-2-macroglobulin receptor chain, reducing ligand binding. LRPAP1 Protein, Human (HEK293, C-His) is the recombinant human-derived LRPAP1 protein, expressed by HEK293 , with C-6*His labeled tag.

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

Immobilized LRPAP1 at 0.5 μg/mL (100 μL/well) can bind Biotinylated VLDLR protein. The ED50 for this effect is 0.5626 nM. Immobilized LRPAP1 at 0.5 μg/mL (100 μL/well) can bind Biotinylated VLDLR protein. The ED50 for this effect is 0.5626 nM.

Alpha-2-macroglobulin receptor-associated protein; Alpha-2-MRAP; RAP; A2MRAP

Human

HEK293

C-6*His

Greater than 95% as determined by SDS-PAGE.

P30533

Y35-L357

YSREKNQPKPSPKRESGEEFRMEKLNQLWEKAQRLHLPPVRLAELHADLKIQERDELAWKKLKLDGLDEDGEKEARLIRNLNVILAKYGLDGKKDARQVTSNSLSGTQEDGLDDPRLEKLWHKAKTSGKFSGEELDKLWREFLHHKEKVHEYNVLLETLSRTEEIHENVISPSDLSDIKGSVLHSRHTELKEKLRSINQGLDRLRRVSHQGYSTEAEFEEPRVIDLWDLAQSANLTDKELEAFREELKHFEAKIEKHNHYQKQLEIAHEKLRHAESVGDGERVSRSREKHALLEGRTKELGYTVKKHLQDLSGRISRARHNEL

Full Length of Mature Protein

42 kDa.

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.