Analytical Data
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Gene name
NIP7
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简介
NIP7 is essential for precise processing of 34S pre-rRNA and assembly of 60S ribosomal subunits. As a monomer, NIP7 interacts with preribosomal complexes, may bind to RNA, and binds to key protein partners, including NOL8, SBDS, and FTSJ3. NIP7 Protein, Human (His) is the recombinant human-derived NIP7 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
60S Ribosome Subunit Biogenesis Protein NIP7 Homolog; KD93; NIP7
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y221-1
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Expression Region
M1-T180
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Protein Length
Full Length of Isoform-1
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Molecular Weight
20 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NIP7, or Nuclear Import Protein 7, is a pivotal component involved in the nuclear transport of proteins, particularly those that play critical roles in various cellular processes. Understanding the mechanisms by which NIP7 functions is essential for elucidating cellular pathways related to gene expression, cell cycle regulation, and response to stress. Research indicates that NIP7 interacts with various nuclear transport receptors to facilitate the import of proteins containing nuclear localization signals (NLS). Furthermore, alterations in NIP7 activity have been linked to several diseases, including cancers and viral infections, where the hijacking of nuclear transport mechanisms can lead to aberrant cellular functioning. The recombinant production of NIP7 has become a focal point for studies aiming to characterize its structural and functional properties, provide insights into its role in disease pathology, and explore its potential as a therapeutic target. By studying NIP7 in a controlled laboratory environment, researchers can manipulate its expression and interactions, leading to a better understanding of its involvement in nuclear import pathways. Consequently, the development of NIP7 as a recombinant protein not only enhances our understanding of nuclear transport dynamics but also opens new avenues for drug discovery and therapeutic interventions in diseases characterized by dysfunctional nuclear transport.
