Analytical Data
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Gene name
IL-34
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简介
IL-34 protein is an important cytokine that promotes the proliferation, survival, and differentiation of monocytes and macrophages. It plays a key role in innate immune responses and inflammatory processes by promoting the release of pro-inflammatory chemokines. IL-34 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IL-34 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Biological Activity
1.Measured in a cell proliferation assay using M-NFS-60 mouse myeloid cells and the ED50 is typically ≤200 ng/mL. 2.Measured by its ability to bind biotinylated recombinant mouse CSF1R in a functional ELISA. Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 11.05 ng/mL. corresponding to a specific activity is 9.04×104 units/mg.
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Alternative Names
Interleukin-34; IL-34; C16orf77
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Species
Mouse
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 95% as determined by reducing SDS-PAGE. as determined by SDS-PAGE.
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Uniprot
Q8R1R4
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Expression Region
Asn21~Pro235
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AA Sequence
NENLEIWTLTQDKECDLTGYLRGKLQYKNRLQYMKHYFPINYRIAVPYEGVLRVANITRLQKAHVSERELRYLWVLVSLNATESVMDVLLEGHPSWKYLQEVQTLLENVQRSLMDVEIGPHVEAVLSLLSTPGLSLKLVRPKALLDNCFRVMELLYCSCCKQSPILKWQDCELPRLHPHSPGSLMQCTATNVYPLSRQTPTSLPGSPSSSHGSLP
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Protein Length
Full Length of Isoform-1 Mature Protein
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Molecular Weight
35-40 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
IL-34 (Interleukin-34) is a cytokine that plays a crucial role in the regulation of immune responses, particularly in the differentiation and survival of macrophages. Discovered in 2008, its unique ability to bind to the colony-stimulating factor 1 receptor (CSF1R) distinguishes it from other monocyte/macrophage growth factors. Research into IL-34 has gained momentum due to its implications in various pathological conditions, including chronic inflammatory diseases, autoimmune disorders, and cancer. The recombinant protein version of IL-34 is a valuable tool for studying its biological functions and mechanisms of action. It can be used to assess the effects of IL-34 on macrophage polarization, migration, and cytokine production, providing insights into its role in both health and disease. Moreover, due to its involvement in the tumor microenvironment, IL-34 is being investigated as a potential therapeutic target for cancer treatment. Understanding the structure, signaling pathways, and functional properties of recombinant IL-34 can lead to the development of novel therapeutic strategies aimed at modulating immune responses in various diseases.
