Analytical Data
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Gene name
Ribonuclease J/RnJ
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简介
Ribonuclease J/RnJ Proteinas, an enzyme with 5'-3' exonuclease activity and potential endonuclease function, is crucial in ribosomal RNA (rRNA) maturation. It also plays a role in mRNA maturation/decay, contributing to intricate RNA processing, ensuring precise maturation, and quality control in the cellular environment. Ribonuclease J/RnJ Protein, Synechocystis sp. (His-SUMO) is the recombinant Ribonuclease J/RnJ protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
- Application
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Alternative Names
rnj; slr0551; Ribonuclease J; RNase J
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Species
Others
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Source
E. coli
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Tag
N-His;N-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P54123
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Expression Region
M1-S640
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AA Sequence
MAKNTQTQALKILPLGGLHEIGKNTCVFEYDDEILLLDAGLAFPTDDMHGVNVVLPDMTYLRENREKIKGMVVTHGHEDHIGGIAYHLKQFDIPIIYGPRLAMALLRDKLEEAGMLERTNLQTVSPREMVRLGKSFVVEFIRNTHSIADSYCLAIHTPLGVVMHSGDFKIDHTPIDGEFFDLQKVAEYGEKGVLCLLSDSTNAEVPGITPSEASVIPNLDRVFSQAEGRLMVTTFASSVHRVNIILSLAQKHQRKVAVVGRSMLNVIAHARKLGYIKCPDNLFVPLKAARNLPDQQQLILTTGSQGEPLAAMTRISNGEHPQIKIRQGDTVVFSANPIPGNTIAVVNTIDRLMMQGANVIYGKHQGIHVSGHASQEEHKMLLALTRPKFFVPVHGEHRMLVKHSQMAQAQGIPSENIVIVNNGDVIELTGDRIRVAGQVPSGIELVDQAGIVHESTMAERQQMAEDGLVTVAAALSKTGTLLAYPEVHCRGVVMTIQPKLLEELIVRTIENFLTERWSEFTHGSNGSTEVSWNALQKELESSLQRLIKRELQSSPMVLLMLQTDTPIELDQVPQNVSTVSATSATPAPRKKVVLTKTPEPKVKAKPEKKVVTTAEPSAQPVSTTKVYRRSRKRSTTSVSS
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Protein Length
Full Length
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Molecular Weight
86.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Ribonuclease J (RnJ) is a key enzyme involved in RNA processing and degradation in various organisms, particularly in bacteria. It plays a crucial role in the maturation of ribosomal RNA and the turnover of messenger RNA, thereby influencing gene expression and regulation. The study of RnJ is of significant interest due to its unique structure and catalytic mechanisms, which differ from other ribonucleases. Understanding RnJ's function can provide insights into bacterial RNA metabolism and the broader implications of RNA biology in cellular processes. Moreover, its potential applications in biotechnology and medicine, such as antibiotic development or RNA-based therapeutics, make it a target for recombinant protein studies. By producing and characterizing recombinant RnJ, researchers aim to investigate its enzymatic properties, substrate specificity, and interaction with other molecules, paving the way for innovative approaches in microbial biology and RNA research. The insights gained from such studies could contribute to our understanding of bacterial pathogenesis and the development of novel antimicrobial strategies.
