Analytical Data
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基因名
TGF beta 1/TGFB1
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简介
TGF beta 1/TGFB1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. TGF beta 1 is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, apoptosis, and can regulate the expression and activation of other growth factors, including interferon gamma and tumor necrosis factor alpha. TGF beta 1/TGFB1 LAP Protein, Human (HEK293) is the recombinant human-derived TGF beta 1/TGFB1 LAP protein, expressed by HEK293 , with tag free. and C33S mutation.
- Application
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生物活性
1.Measured by its ability to inhibit TGF-β1 activity on HT-2 mouse T cells. The ED50 this effect is 20-100 ng/mL in the presence of 1 ng/mL TGF-β1. 2.Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells.The ED50 for this effect is 0.01185 ng/ml, corresponding to a specific activity is 8.43×104 units/mg. Measured by its ability to inhibit TGF-β1 activity on HT-2 mouse T cells. The ED50 this effect is 22.78 ng/mL in the presence of 1 ng/mL TGF-β1, corresponding to a specific activity is 4.39×104 units/mg.
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别名
Transforming Growth Factor Beta-1; TGF-Beta-1; Latency-Associated Peptide; LAP; TGFB1; TGFB
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种属
Human
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表达系统
HEK293
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标签
Tag Free
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纯度
Greater than 95% as determined by reducing SDS-PAGE.
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蛋白编号
P01137
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表达区间
L30-R278, C33S
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氨基酸序列
LSTSKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRR
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蛋白长度
Full Length of Latency-associated Peptide
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分子量
20-45 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Transforming Growth Factor Beta 1 (TGF-β1) is a multifunctional cytokine that plays a critical role in various cellular processes, including proliferation, differentiation, and immune response regulation. Dysregulation of TGF-β1 signaling is implicated in numerous diseases, including cancer, fibrosis, and autoimmune disorders. Research on recombinant TGF-β1 (rTGF-β1) has gained significant attention due to its potential therapeutic applications. By using recombinant DNA technology, researchers can produce high-purity rTGF-β1 for in vitro and in vivo studies. These studies not only help elucidate the molecular mechanisms underlying TGF-β1-mediated pathways but also provide insights into its role in pathological conditions. Furthermore, rTGF-β1 has been explored as a therapeutic agent in tissue engineering and regenerative medicine, particularly in promoting wound healing and tissue repair. However, challenges such as controlling TGF-β1's pleiotropic effects and ensuring tissue-specific responses still need to be addressed. Overall, the characterization and application of rTGF-β1 represent a promising area of research with significant implications for understanding TGF-β1's role in health and disease, as well as opening avenues for novel therapeutic interventions.
