Recombinant Human Fibrillin-1/Asprosin Protein (HEK293)，His

Fibrillin-1/Asprosin

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

1.Measured by its binding ability in a functional ELISA. Immobilized Human MFAP4 at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human Fibrillin-1. The ED50 for this effect is ≤0.8473 μg/mL, corresponding to a specific activity is ≥1.18×103 Unit/mg. 2.Measured by its ability to induce TNF-ɑ secretion by macrophages produced by THP-1 cells treated with dihydroxyvitamin D3. Measured by its binding ability in a functional ELISA. Immobilized Human MFAP4 at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human Fibrillin-1. The ED50 for this effect is 0.6163 μg/mL, corresponding to a specific activity is 1.62×103 Unit/mg.

rHuAsprosin, His; Fibrillin-1; FBN1; Asprosin; FBN

Human

HEK293

N-His

Greater than 95% as determined by SDS-PAGE.

P35555

S2732-H2871

Partial

25-33 kDa.

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.