Analytical Data
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Gene name
MetC
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简介
MetC protein is a multifunctional enzyme that catalyzes the cleavage of cystathionine to homocysteine, pyruvate, and ammonia, which is essential for methionine biosynthesis. It also acts as a cysteine desulfurase, converting cysteine into sulfide. MetC Protein, E.coli (His) is the recombinant E. coli-derived MetC protein, expressed by E. coli , with N-His labeled tag.
- Application
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Alternative Names
metC; b3008; JW2975Cystathionine beta-lyase MetC; CBL; CL; CD; EC 4.4.1.28; Cysteine lyase MetC; Cysteine-S-conjugate beta-lyase MetC
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Species
E.coli
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P06721
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Expression Region
M1-A392
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AA Sequence
MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKHATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFIEQGDHVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSITMEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDAMIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEWLAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLFSMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MetC, a critical component in bacterial metabolism and a key player in various biochemical pathways, has garnered significant attention in recent years due to its potential applications in biotechnology and medicine. The research on MetC recombinant proteins is primarily driven by the need to better understand its role in cellular processes and its interactions with other proteins. The unique properties of MetC, including its enzymatic activity and stability under varying conditions, make it an ideal candidate for recombinant expression systems. By utilizing techniques such as molecular cloning and protein engineering, scientists aim to produce MetC in high yields, facilitating the study of its functional mechanisms. Furthermore, recombinant MetC proteins can be utilized in the development of novel therapeutic approaches, including drug delivery systems and targeted therapies. As the field of synthetic biology advances, the capability to design and manipulate MetC can lead to innovative solutions in combating bacterial infections and enhancing industrial bioprocesses. Consequently, ongoing research focuses on optimizing production strategies, characterizing the protein's structure and function, and exploring its applications in various fields, paving the way for future advancements in protein-based technologies.
