Analytical Data
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基因名
Grb2
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简介
Grb2, an adapter protein, crucially connects cell surface growth factor receptors to the Ras signaling pathway. Despite no direct binding to phosphorylated EGFR, Grb2 inhibits EGF-induced transactivation of a RAS-responsive element. It acts as a dominant negative relative to GRB2, suppressing proliferative signals and potentially initiating programmed cell death. Grb2 Protein, Human (His) is the recombinant human-derived Grb2 protein, expressed by E. coli , with C-6*His labeled tag.
- Application
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生物活性
1.Immobilized Human GRB2 at 2 μg/mL (100 μL/well) can bind Anti-GRB2 Antibody. The ED50 for this effect is 93.39 ng/mL. 2.Measured in a cell proliferation assay using A549 cells. The ED50 for this effect is ≤33.3 ng/mL, corresponding to a specific activity is 3.003×104 units/mg Measured in a cell proliferation assay using A549 cells. The ED50 for this effect is 33.3 ng/mL, corresponding to a specific activity is 3.003×104 units/mg.
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别名
rHuGrowth factor receptor-bound protein 2/GRB2, His; Growth Factor Receptor-Bound Protein 2; Adapter Protein GRB2; Protein Ash; SH2/SH3 Adapter GRB2; GRB2; ASH
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种属
Human
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表达系统
E. coli
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标签
C-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
P62993-1
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表达区间
M1-V217
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氨基酸序列
MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV
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蛋白长度
Full Length of Isoform-1
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分子量
25-29 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Grb2 (Growth factor receptor-bound protein 2) is a key adaptor protein involved in various cellular signaling pathways, particularly those associated with growth factor receptor activation. Its primary role is to mediate signal transduction from receptor tyrosine kinases (RTKs) to downstream effectors, including the Ras-MAPK pathway, which is crucial for cellular processes such as proliferation, differentiation, and survival. Research into Grb2 recombinant protein has gained significant attention due to its central role in oncogenesis and various diseases, including cancer. The ability to produce Grb2 as a recombinant protein allows for detailed biochemical characterization, including studies on its binding affinities and interaction with other signaling molecules. Additionally, understanding the structural dynamics of Grb2 through methods such as X-ray crystallography or NMR spectroscopy can provide insights into its functional mechanisms. Furthermore, the development of Grb2 inhibitors or modulators holds therapeutic potential, particularly in targeting cancers that exhibit aberrant RTK signaling. Thus, the study of Grb2 recombinant protein is essential for elucidating its role in cellular signaling and for advancing potential therapeutic strategies aimed at diseases related to dysregulated signaling pathways. This research can ultimately contribute to the identification of novel drug targets, enhancing our understanding of cellular communications and pathophysiological conditions associated with Grb2 activity.
