Analytical Data
-
基因名
Alpha-Fetoprotein
- Application
-
别名
rHuAlpha-Fetoprotein, His; AFP; Alpha-Fetoprotein
-
种属
Human
-
表达系统
HEK293
-
标签
C-6*His
-
纯度
Greater than 95% as determined by SDS-PAGE.
-
蛋白编号
P02771
-
表达区间
T20-V609
-
氨基酸序列
TLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSLLNQHACAVMKNFGTRTFQAITVTKLSQKFTKVNFTEIQKLVLDVAHVHEHCCRGDVLDCLQDGEKIMSYICSQQDTLSNKITECCKLTTLERGQCIIHAENDEKPEGLSPNLNRFLGDRDFNQFSSGEKNIFLASFVHEYSRRHPQLAVSVILRVAKGYQELLEKCFQTENPLECQDKGEEELQKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV
-
蛋白长度
Full Length of Mature Protein
-
分子量
60-70 kDa.
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Alpha-fetoprotein (AFP) is a glycoprotein produced predominantly in the fetal liver, yolk sac, and gastrointestinal tract. It serves various biological functions, including acting as a transport protein for fatty acids and playing a role in immune modulation. Beyond its physiological roles in development, AFP levels are also clinically significant, as they are used as a tumor marker, particularly in hepatocellular carcinoma (HCC) and germ cell tumors. Research has increasingly focused on the recombinant production of AFP, driven by the need for standardized quantities of the protein for diagnostics and therapeutic applications. The development of recombinant AFP not only facilitates improved understanding of its structure-function relationships but also aids in the creation of novel cancer immunotherapies and vaccine formulations. Moreover, the ability to engineer AFP allows scientists to explore its potential role in drug delivery systems and peptide-based imaging agents. Achieving high-yield expression systems, coupled with effective purification methods, remains a critical area of study to ensure the availability of this biomarker for clinical and research purposes. Thus, the investigation into the recombinant production of AFP embodies a multidisciplinary approach, bridging molecular biology, biochemistry, and clinical applications, emphasizing its significance in advancing cancer research and improving patient outcomes.
