Analytical Data
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基因名
Periostin
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简介
Periostin protein, a multifaceted regulator, induces cell attachment and spreading, participates in cell adhesion, and enhances BMP1 incorporation into the fibronectin matrix. It forms a structural homodimer and interacts with BMP1 and fibronectin. This interplay positions Periostin as a key player in regulating cellular and tissue functions, including cell adhesion, tissue architecture, and extracellular matrix modulation. Periostin Protein, Human (758a.a, HEK293, His) is the recombinant human-derived Periostin protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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生物活性
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 2.456 μg /mL, corresponding to a specific activity is 407.17 units/mg. Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 2.456 μg /mL, corresponding to a specific activity is 407.17 units/mg.
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别名
Osteoblast-specific factor 2; OSF-2; POSTN; Periostin
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种属
Human
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q15063-2
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表达区间
N22-Q779
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氨基酸序列
NNHYDKILAHSRIRGRDQGPNVCALQQILGTKKKYFSTCKNWYKKSICGQKTTVLYECCPGYMRMEGMKGCPAVLPIDHVYGTLGIVGATTTQRYSDASKLREEIEGKGSFTYFAPSNEAWDNLDSDIRRGLESNVNVELLNALHSHMINKRMLTKDLKNGMIIPSMYNNLGLFINHYPNGVVTVNCARIIHGNQIATNGVVHVIDRVLTQIGTSIQDFIEAEDDLSSFRAAAITSDILEALGRDGHFTLFAPTNEAFEKLPRGVLERIMGDKVASEALMKYHILNTLQCSESIMGGAVFETLEGNTIEIGCDGDSITVNGIKMVNKKDIVTNNGVIHLIDQVLIPDSAKQVIELAGKQQTTFTDLVAQLGLASALRPDGEYTLLAPVNNAFSDDTLSMDQRLLKLILQNHILKVKVGLNELYNGQILETIGGKQLRVFVYRTAVCIENSCMEKGSKQGRNGAIHIFREIIKPAEKSLHEKLKQDKRFSTFLSLLEAADLKELLTQPGDWTLFVPTNDAFKGMTSEEKEILIRDKNALQNIILYHLTPGVFIGKGFEPGVTNILKTTQGSKIFLKEVNDTLLVNELKSKESDIMTTNGVIHVVDKLLYPADTPVGNDQLLEILNKLIKYIQIKFVRGSTFKEIPVTVYKPIIKKYTKIIDGVPVEITEKETREERIITGPEIKYTRISTGGGETEETLKKLLQEEVTKVTKFIEGGDGHLFEDEEIKRLLQGDTPVRKLQANKKVQGSRRRLREGRSQ
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蛋白长度
Partial
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分子量
85-90 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Periostin, a matricellular protein, plays a crucial role in various biological processes, including tissue development, repair, and inflammatory responses. Initially identified as a key component of the periosteum, its expression has been observed in numerous tissues, particularly during embryogenesis and in response to injury. Research has indicated that periostin is involved in cell adhesion, migration, and survival, mediated through its interactions with integrins and other extracellular matrix components. This protein has garnered significant attention in the context of several diseases, including fibrosis, cardiovascular disorders, and cancer, where its upregulation correlates with disease progression and poor prognosis. The recombinant form of periostin has emerged as a valuable tool for studying its functional roles in vitro and in vivo, enabling researchers to dissect its mechanistic pathways and therapeutic potentials. Additionally, periostin's role as a biomarker for certain conditions has prompted investigations into its utility in clinical settings, paving the way for novel diagnostic and therapeutic strategies. The ongoing research into periostin's biological functions underscores its significance in health and disease, emphasizing the necessity for further exploration of this versatile protein and its potential applications in regenerative medicine and targeted therapies.
