Analytical Data
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基因名
ADAM15
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简介
ADAM15 is an active metalloprotease that plays a crucial role in physiological processes and has gelatin-solubilizing and collagen-solubilizing activities. Notably, it affects wound healing, mediates intraepithelial cell/T cell interactions, and inhibits β-1 integrin-mediated cell adhesion and migration in airway smooth muscle cells. ADAM15 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ADAM15 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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生物活性
Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 4.53 μg/mL, corresponding to a specific activity is 2.21×102 units/mg. Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 4.53 μg/mL， corresponding to a specific activity is 2.21×102 units/mg.
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别名
Disintegrin and metalloproteinase domain-containing protein 15; ADAM 15; MDC-15; Metargidin
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种属
Mouse
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表达系统
HEK293
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标签
C-10*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
O88839
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表达区间
R18-T697
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氨基酸序列
RPSPPLPNIGGTEEEQQASPERTLSGSMESRVVQDSPPMSLADVLQTGLPEALRISLELDSESHVLELLQNRDLIPGRPTLVWYQPDGTRMVSEGYSLENCCYRGRVQGHPSSWVSLCACSGIRGLIVLSPERGYTLELGPGDLQRPVISRIQDHLLLGHTCAPSWHASVPTRAGPDLLLEQHHAHRLKRDVVTETKIVELVIVADNSEVRKYPDFQQLLNRTLEAALLLDTFFQPLNVRVALVGLEAWTQHNLIEMSSNPAVLLDNFLRWRRTDLLPRLPHDSAQLVTVTSFSGPMVGMAIQNSICSPDFSGGVNMDHSTSILGVASSIAHELGHSLGLDHDSPGHSCPCPGPAPAKSCIMEASTDFLPGLNFSNCSRQALEKALLEGMGSCLFERQPSLAPMSSLCGNMFVDPGEQCDCGFPDECTDPCCDHFTCQLRPGAQCASDGPCCQNCKLHPAGWLCRPPTDDCDLPEFCPGDSSQCPSDIRLGDGEPCASGEAVCMHGRCASYARQCQSLWGPGAQPAAPLCLQTANTRGNAFGSCGRSPGGSYMPCAPRDVMCGQLQCQWGRSQPLLGSVQDRLSEVLEANGTQLNCSWVDLDLGNDVAQPLLALPGTACGPGLVCIGHRCQPVDLLGAQECRRKCHGHGVCDSSGHCRCEEGWAPPDCMTQLKATSSLTT
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蛋白长度
Extracellular Domain (with Propeptide)
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分子量
75-110 kDa.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ADAM15, a member of the ADAM (A Disintegrin and Metalloproteinase) family, plays a critical role in various biological processes, including cell adhesion, migration, and proteolytic activity. This protein is characterized by its ability to shed membrane-bound proteins, influencing signaling pathways and modulating interactions between cells and their microenvironment. Research has highlighted ADAM15's involvement in pathological conditions such as cancer metastasis, inflammation, and cardiovascular diseases, making it a potential therapeutic target. The need for recombinant ADAM15 protein arises from the necessity to study its structure-function relationships, discover its interacting partners, and elucidate its molecular mechanisms in various cellular contexts. Moreover, recombinant ADAM15 can serve as a valuable tool for drug development and the exploration of its role in disease progression. By generating a stable, functional form of this protein, researchers aim to investigate ADAM15's enzymatic activity, structural properties, and its regulatory effects on key signaling pathways, paving the way for novel therapeutic interventions in diseases associated with dysregulated ADAM15 activity. This research underscores the importance of understanding the multifaceted roles of ADAM15 in health and disease, facilitating the development of targeted therapies that can modulate its function for clinical benefit.
