Analytical Data
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Gene name
BMP-4
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简介
Bone morphogenetic protein 4 (BMP-4) is a polymorphic ligand protein belonging to the TGF-β family, which is involved in the circulation of the vascular system and can activate receptors on vascular cells[1]. BMP-4 binds to type I receptors (ALK-2/-3/-6) and type II receptors (BMPR2, ACVR2A)[2] to increase plaque formation and promote oxidative stress, endothelial dysfunction, and osteogenic differentiation through its pro-inflammatory and pro-atherogenic effects[3]. BMP-4 Protein, Mouse (HEK293, Fc) has a total length of 116 amino acids (S293-R408), is expressed in HEK293 cells with N-terminal rabbit Fc-tag.
- Application
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Biological Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 11.22 ng/mL, corresponding to a specific activity is 8.913×104 U/mg. Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED50 for this effect is 11.22 ng/mL, corresponding to a specific activity is 8.913×104 U/mg.
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Alternative Names
BMP-2B; BMP-4; Bone morphogenetic protein 4; DVR4
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Species
Mouse
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Source
HEK293
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Tag
N-rFc
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
P21275
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Expression Region
S293-R408
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Protein Length
Full Length of Mature Protein
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Molecular Weight
45-50 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Bone Morphogenetic Protein 4 (BMP-4) is a member of the transforming growth factor-beta (TGF-β) superfamily, known for its crucial role in regulating embryonic development, particularly in bone and cartilage formation. Research on BMP-4 has gained momentum due to its significant implications in various biological processes, including mesenchymal stem cell differentiation, tissue regeneration, and repair, as well as its involvement in pathological conditions like cancer and degenerative diseases. BMP-4 functions primarily through activating specific receptors on target cells, leading to a cascade of intracellular signaling pathways that influence gene expression. The recombinant production of BMP-4 has facilitated extensive studies aimed at understanding its mechanism of action and therapeutic potential. Researchers have developed various recombinant forms of BMP-4, enabling investigations into its precise effects on skeletal tissues and its utility in clinical applications, such as enhancing bone healing in orthopedic surgeries and treating bone-related disorders. Furthermore, studies have delved into the molecular interactions of BMP-4 with other signaling pathways, underscoring its role in a complex network of growth factors. Overall, the ongoing exploration of BMP-4, particularly its recombinant protein forms, continues to shed light on its biological significance and pave the way for innovative therapeutic strategies in regenerative medicine.
