Analytical Data
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Gene name
ATG9B
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简介
ATG9B Protein, a phospholipid scramblase crucial for autophagy, dynamically cycles between the preautophagosomal structure (PAS) and the cytoplasmic vesicle pool. It plays a critical role in expanding autophagosomal membranes, facilitating lipid distribution with ATG2 and driving membrane expansion. Additionally, ATG9B participates in necrotic cell death. ATG9B Protein, Human (HEK293, FLAG) is the recombinant human-derived ATG9B protein, expressed by HEK293 , with C-Flag labeled tag.
- Application
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Alternative Names
ATG9B; Autophagy-related protein 9B; APG9-like 2; Nitric oxide synthase 3-overlapping antisense gene protein; Protein sONE
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Species
Human
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Source
HEK293
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Tag
C-Flag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q674R7
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Expression Region
M1-D924
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Protein Length
Full Length
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ATG9B, a member of the ATG (autophagy-related) protein family, plays a crucial role in the regulation of autophagy, a vital cellular process involved in degradation and recycling of cellular components. Unlike other ATG proteins that are primarily localized in the cytoplasm, ATG9B is unique due to its distinct membrane-associated function, facilitating the transport of lipid membranes necessary for autophagosome formation. Research has shown that ATG9B is involved in various physiological processes, such as cell differentiation, immune responses, and the removal of damaged organelles, linking its activity to multiple diseases, including neurodegenerative disorders and cancer. Given its pivotal role in autophagy, studying the recombinant expression of ATG9B offers significant insights into understanding its mechanism, interaction with other proteins, and overall contribution to autophagy regulation. This research can further provide therapeutic targets for autophagy-related diseases, enhancing our ability to design interventions for conditions where autophagy is dysregulated. Therefore, the exploration of ATG9B recombinant protein not only contributes to the fundamental biology of autophagy but also holds potential for clinical applications in treating a variety of pathologies.
