Analytical Data
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Gene name
TRPA1
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简介
The TRPA1 protein is a receptor-activated nonselective cation channel that is critical for pain detection and may affect cold sensation, oxygen sensing, cough reflex, itch, and inner ear function. It responds to inflammatory mediators and irritants such as allyl thiocyanate (AITC), cinnamic aldehyde, diallyl disulfide (DADS), and acrolein. TRPA1 Protein, Human (HEK293, His, MBP, FLAG) is the recombinant human-derived TRPA1 protein, expressed by HEK293 , with N-10*His, N-MBP, C-Flag labeled tag.
- Application
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Alternative Names
TRPA1; Transient receptor potential cation channel subfamily A member 1; Ankyrin-like with transmembrane domains protein 1; Transformation-sensitive protein p120; Wasabi receptor
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Species
Human
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Source
HEK293
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Tag
N-MBP;C-Flag;N-10*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O75762
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Expression Region
K2-P1119
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Protein Length
Full Length
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Transient Receptor Potential A1 (TRPA1) is a cation channel primarily expressed in sensory neurons and plays a crucial role in nociception and thermosensation. It is activated by various stimuli, including environmental irritants, harmful substances, and temperature extremes, making it a key player in pain perception and inflammatory responses. Due to its involvement in pain mechanisms, TRPA1 has garnered significant interest in the field of pain research and pharmacology, with the potential for therapeutic interventions targeting this channel. The recombinant expression of TRPA1 allows for detailed investigation of its biophysical properties, activation mechanisms, and pharmacological modulation. Research into TRPA1 has been propelled by advances in molecular biology techniques, enabling the production of functional TRPA1 proteins for in vitro studies. These recombinant proteins facilitate high-throughput screening of novel analgesics and contribute to a deeper understanding of TRPA1's role in pathological conditions associated with chronic pain, neuropathy, and inflammatory diseases. Overall, the study of TRPA1 recombinant proteins holds promise for developing targeted strategies to alleviate pain and improve patient outcomes in various pain-related disorders.
