Analytical Data
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Gene name
GMNN
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简介
GMNN proteins are important inhibitors of DNA replication and prevent the incorporation of MCM complexes into prereplication complexes. During mitosis, GMNN undergoes degradation, enabling replication in subsequent cell cycles. GMNN Protein, Human (His, Strep) is the recombinant human-derived GMNN protein, expressed by E. coli , with N-Strep, N-6*His labeled tag.
- Application
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Alternative Names
GMNN; Geminin
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Species
Human
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Source
E. coli
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Tag
N-StrepⅡ;N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O75496
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Expression Region
M1-I209
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Protein Length
Full Length
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GMNN (Geminin) is a crucial protein involved in the regulation of DNA replication and cell cycle control. Its primary role is to inhibit the licensing of DNA replication origins, thereby preventing re-replication and ensuring that each segment of the genome is replicated only once during the S phase of the cell cycle. Dysregulation of GMNN expression has been linked to various cancers, making it a significant target for therapeutic interventions. In recent years, the study of GMNN and its recombinant forms has gained attention, as researchers aim to elucidate its structural properties and functional mechanisms. By producing GMNN as a recombinant protein, scientists can investigate its interactions with other cell cycle regulators and DNA replication machinery in detail. Understanding these interactions is vital for developing novel cancer treatments that specifically target the pathways influenced by GMNN. Additionally, the recombinant GMNN can be used as a tool in various biochemical assays to explore its role in cellular processes beyond replication, including transcription regulation and response to DNA damage. Thus, the research on GMNN, particularly its recombinant forms, serves as a significant foundation for uncovering critical aspects of cell cycle regulation and its implications in cancer biology.
