Analytical Data
-
Gene name
CRKL
-
简介
CRKL protein mediates intracellular signal transduction, interacts with tyrosine-phosphorylated EPOR and INPP5D/SHIP1, and plays a role in erythropoietin receptor and phosphoinositide signaling. It also binds to DOCK2 and DOCK5, suggesting regulation of guanine nucleotide exchange factors. CRKL Protein, Human (His, Strep) is the recombinant human-derived CRKL protein, expressed by E. coli , with N-Strep, N-6*His labeled tag.
- Application
-
Alternative Names
CRKL; Crk-like protein
-
Species
Human
-
Source
E. coli
-
Tag
N-StrepⅡ;N-6*His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P46109
-
Expression Region
M1-E303
-
Protein Length
Full Length
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CRKL (Crk-like protein) is a pivotal adaptor protein implicated in various cellular processes, including signal transduction, cytoskeletal organization, and cell migration. It's a member of the Crk family of proteins, which are characterized by SH2 (Src Homology 2) and SH3 (Src Homology 3) domains that facilitate interaction with diverse signaling molecules. The study of CRKL has gained significance due to its role in cancer biology, particularly in connection with the phosphorylation-dependent signaling pathways influenced by receptor tyrosine kinases and oncogenic proteins. Dysregulation of CRKL has been linked to several malignancies, suggesting that targeting CRKL may offer therapeutic potential. Recent advancements in recombinant protein technology have enabled the production of purified CRKL for biochemical and structural studies, providing insights into its functional mechanisms and interactions in cellular contexts. Understanding the structural dynamics and functional roles of CRKL can elucidate its contribution to oncogenic signaling, advance the development of CRKL-targeted therapies, and improve therapeutic strategies in cancer treatment. Hence, ongoing research focuses on the detailed characterization of CRKL, aiming to uncover novel regulatory pathways and interactions that may be harnessed for clinical applications in oncology.
