Analytical Data
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Gene name
OPN1LW
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简介
OPN1LW protein, crucial for vision, is a vital element of visual pigments responsible for light absorption. The pigments consist of opsin, forming a covalent bond with cis-retinal. This molecular mechanism is indispensable for perceiving light stimuli in vision. OPN1LW Protein, Human (Cell-Free, His) is the recombinant human-derived OPN1LW protein, expressed by E. coli Cell-free , with N-10*His labeled tag.
- Application
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Alternative Names
Long-wave-sensitive opsin 1; Red cone photoreceptor pigment; Red-sensitive opsin; ROP
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Species
Human
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Source
E. coli Cell-free
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Tag
N-10*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P04000
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Expression Region
M1-A364
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AA Sequence
MAQQWSLQRLAGRHPQDSYEDSTQSSIFTYTNSNSTRGPFEGPNYHIAPRWVYHLTSVWMIFVVTASVFTNGLVLAATMKFKKLRHPLNWILVNLAVADLAETVIASTISIVNQVSGYFVLGHPMCVLEGYTVSLCGITGLWSLAIISWERWLVVCKPFGNVRFDAKLAIVGIAFSWIWSAVWTAPPIFGWSRYWPHGLKTSCGPDVFSGSSYPGVQSYMIVLMVTCCIIPLAIIMLCYLQVWLAIRAVAKQQKESESTQKAEKEVTRMVVVMIFAYCVCWGPYTFFACFAAANPGYAFHPLMAALPAYFAKSATIYNPVIYVFMNRQFRNCILQLFGKKVDDGSELSSASKTEVSSVSSVSPA
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Protein Length
Full Length
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
OPN1LW, also known as the long-wavelength-sensitive opsin, is a crucial photopigment found in the cone cells of the retina, specifically responsible for sensing long wavelengths of light, which correspond to the color red. Research into OPN1LW is primarily driven by its significance in color vision, as it plays a fundamental role in how humans perceive a broad spectrum of colors. Mutations or dysfunctions in the OPN1LW gene can lead to various forms of color blindness, particularly red-green color blindness, which affects a significant portion of the population. Furthermore, understanding the structure and function of OPN1LW can provide insights into the molecular mechanisms underlying phototransduction—the process by which light is converted into neural signals. Recent advancements in recombinant protein technology have enabled scientists to produce and analyze OPN1LW at a molecular level, facilitating in-depth studies of its binding properties, stability, and interactions with other cellular components. This research not only enhances our understanding of visual biology but also has potential applications in developing gene therapies and corrective treatments for color vision deficiencies, making OPN1LW a key target for studies in both basic and applied sciences.
