Analytical Data
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Gene name
EIF4A1
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简介
The EIF4A1 protein is an ATP-dependent RNA helicase and an important subunit of the eIF4F complex, which is essential for cap recognition and promoting the binding of mRNA to ribosomes. In the translation initiation model, EIF4A1 helps unravel RNA secondary structure in the mRNA 5'-UTR. EIF4A1 Protein, Human (His, Strep) is the recombinant human-derived EIF4A1 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag.
- Application
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Alternative Names
EIF4A1; Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1
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Species
Human
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Source
E. coli
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Tag
N-StrepⅡ;N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P60842
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Expression Region
M1-I406
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Protein Length
Full Length
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Molecular Weight
49.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EIF4A1, a member of the eukaryotic translation initiation factor family, is a crucial RNA helicase that plays a key role in the initiation of protein synthesis. It unwinds mRNA secondary structures, promoting the formation of the translation initiation complex and ensuring the proper assembly of ribosomes. Abnormalities in EIF4A1 function have been linked to various diseases, including cancer, neurodegenerative disorders, and viral infections, as these conditions often exploit the host's translational machinery for their own replication and survival. The study of recombinant EIF4A1 proteins provides insights into their enzymatic functions, structural properties, and interactions with other translation factors and RNA molecules. Such research is essential for developing targeted therapeutic strategies, as inhibiting or modulating EIF4A1 activity could potentially disrupt the protein synthesis pathways exploited by pathogenic organisms or malignant cells. Furthermore, understanding the molecular mechanisms governing EIF4A1's role in translation can contribute to the broader field of gene expression regulation and may unveil novel biological processes critical for cellular homeostasis. Overall, investigations into EIF4A1 recombinant proteins serve as a foundation for advancing our knowledge of translation regulation and its implications in health and disease.
