Recombinant Human INHBB Protein(HEK293), C-10*His

INHBB

The INHBB protein complexly controls pituitary function, regulating follicle-stimulating hormone and activin. Its critical role extends to a variety of physiological processes, including hormone secretion, cell development, insulin release, and bone growth. INHBB Protein, Human (HEK293, His) is the recombinant human-derived INHBB protein, expressed by HEK293 , with C-10*His labeled tag.

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

1.Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 this effect is 0.6762 ng/mL, corresponding to a specific activity is 1.48×106 units/mg. (Note: Protein mainly exists in propeptide form, which means it doesn't have the biological activity of Activin B.) 2.Measured by its binding ability in a functional ELISA.Immobilized human INHBB-His at 10 μg/mL (100 μl/well) can bind biotinylated mouse FLRG-His with a linear range of 0.031-0.156 μg/mL. Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 this effect is 0.6762 ng/mL, corresponding to a specific activity is 1.48×106 units/mg.

Activin beta-B chain; INHBB; Inhibin beta B chain

Human

HEK293

C-10*His

Greater than 95% as determined by reducing SDS-PAGE

P09529

S29-A407

SPTPPPTPAAPPPPPPPGSPGGSQDTCTSCGGFRRPEELGRVDGDFLEAVKRHILSRLQMRGRPNITHAVPKAAMVTALRKLHAGKVREDGRVEIPHLDGHASPGADGQERVSEIISFAETDGLASSRVRLYFFISNEGNQNLFVVQASLWLYLKLLPYVLEKGSRRKVRVKVYFQEQGHGDRWNMVEKRVDLKRSGWHTFPLTEAIQALFERGERRLNLDVQCDSCQELAVVPVFVDPGEESHRPFVVVQARLGDSRHRIRKRGLECDGRTNLCCRQQFFIDFRLIGWNDWIIAPTGYYGNYCEGSCPAYLAGVPGSASSFHTAVVNQYRMRGLNPGTVNSCCIPTKLSTMSMLYFDDEYNIVKRDVPNMIVEECGCA

Full Length of Mature Protein

14-17 kDa (propeptide) & 40-44 kDa (mature form) & 54-58 kDa (proform)

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.