Analytical Data
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基因名
FOXP3
- Application
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别名
FOX-P3; AIID; DIETER; IPEX; JM2; PIDX; XPID; SCURFIN; Forkhead Box Protein P3; Immune Dysregulation,Polyendocrinopathy,Enteropathy,X-Linked
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种属
Human
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表达系统
E. coli
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标签
N-terminal His Tag
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q9BZS1
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表达区间
191~412aa
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氨基酸序列
YPLLANGVCK WPGCEKVFEE PEDFLKHCQA DHLLDEKGRA QCLLQREMVQ SLEQQLVLEKEKLSAMOAHL AGKMALTKAS SVASSDKGSC CIVAAGSQGP VVPAWSGPREAPDSLFAVRR HLWGSHGNST FPEFLHNMDY FKFHNMRPPF TYATLIRWAILEAPEKORTL NEIYHWFTRM FAFFRNHPAT WKNAIRHNLS LHKCFVRVESEKGAVWTVDE LE
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分子量
29kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
FOXP3 (Forkhead box P3) is a critical transcription factor predominantly expressed in regulatory T cells (Tregs), playing a vital role in maintaining immune homeostasis and preventing autoimmune diseases. Mutations in the FOXP3 gene are linked to autoimmune disorders such as IPEX syndrome (Immune dysregulation, Polyendocrinopathy, Enteropathy, X-linked syndrome), characterized by severe immune dysregulation. Understanding the structure and function of FOXP3 is essential for elucidating its role in immune regulation. Research on recombinant FOXP3 protein has gained traction as it allows scientists to dissect its functional domains, post-translational modifications, and interactions with other signaling molecules. The reconstitution of FOXP3 as a recombinant protein facilitates in vitro studies on its stability, DNA-binding capacity, and influence on T cell differentiation. Moreover, investigating FOXP3’s interactions with various co-factors provides insights into its regulatory mechanisms. This research not only enhances our understanding of Treg biology but also opens avenues for therapeutic interventions in autoimmune diseases and cancer, where modulation of Treg activity may improve patient outcomes. Overall, the study of recombinant FOXP3 protein is pivotal for both basic immunology and clinical applications.
