Analytical Data
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Gene name
FOXP3
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简介
FOXP3 is a key transcriptional regulator essential for the development and function of regulatory T cells (Tregs), ensuring immune system homeostasis. It acts as a dual repressor and activator, regulating Treg suppressive function and stability while affecting conventional T cell expansion. FOXP3 Protein, Human (His) is the recombinant human-derived FOXP3 protein, expressed by E. coli , with N-His labeled tag.
- Application
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BZS1-1
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Expression Region
M1-L260
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AA Sequence
MPNPRPGKPSAPSLALGPSPGASPSWRAAPKASDLLGARGPGGTFQGRDLRGGAHASSSSLNPMPPSQLQLPTLPLVMVAPSGARLGPLPHLQALLQDRPHFMHQLSTVDAHARTPVLQVHPLESPAMISLTPPTTATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPNPSAPRKDSTLSAVPQSSYPLLANGVCKWPGCEKVFEEPEDFLKHCQADHLLDEKGRAQCLLQREMVQSLEQQLVLEKEKLSAMQAHL
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Protein Length
Partial
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Molecular Weight
31.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
FOXP3 (Forkhead box P3) is a critical transcription factor predominantly expressed in regulatory T cells (Tregs), playing a vital role in maintaining immune homeostasis and preventing autoimmune diseases. Mutations in the FOXP3 gene are linked to autoimmune disorders such as IPEX syndrome (Immune dysregulation, Polyendocrinopathy, Enteropathy, X-linked syndrome), characterized by severe immune dysregulation. Understanding the structure and function of FOXP3 is essential for elucidating its role in immune regulation. Research on recombinant FOXP3 protein has gained traction as it allows scientists to dissect its functional domains, post-translational modifications, and interactions with other signaling molecules. The reconstitution of FOXP3 as a recombinant protein facilitates in vitro studies on its stability, DNA-binding capacity, and influence on T cell differentiation. Moreover, investigating FOXP3’s interactions with various co-factors provides insights into its regulatory mechanisms. This research not only enhances our understanding of Treg biology but also opens avenues for therapeutic interventions in autoimmune diseases and cancer, where modulation of Treg activity may improve patient outcomes. Overall, the study of recombinant FOXP3 protein is pivotal for both basic immunology and clinical applications.
