Analytical Data
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Gene name
PRMT3
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简介
PRMT3 protein is a multifunctional protein arginine N-methyltransferase. As a type I methyltransferase, it catalyzes monomethylation and asymmetric dimethylation of arginine residues in target proteins. In addition to its methyltransferase role, PRMT3 may also regulate retinoic acid synthesis and signaling by inhibiting ALDH1A1 retinal dehydrogenase activity. PRMT3 Protein, Human (His) is the recombinant human-derived PRMT3 protein, expressed by E. coli , with N-10*His labeled tag.
- Application
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Alternative Names
Protein arginine N-methyltransferase 3; PRMT3; HRMT1L3
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Species
Human
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Source
E. coli
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Tag
N-10*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
O60678-1
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Expression Region
C2-Q531
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AA Sequence
CSLASGATGGRGAVENEEDLPELSDSGDEAAWEDEDDADLPHGKQQTPCLFCNRLFTSAEETFSHCKSEHQFNIDSMVHKHGLEFYGYIKLINFIRLKNPTVEYMNSIYNPVPWEKEEYLKPVLEDDLLLQFDVEDLYEPVSVPFSYPNGLSENTSVVEKLKHMEARALSAEAALARAREDLQKMKQFAQDFVMHTDVRTCSSSTSVIADLQEDEDGVYFSSYGHYGIHEEMLKDKIRTESYRDFIYQNPHIFKDKVVLDVGCGTGILSMFAAKAGAKKVLGVDQSEILYQAMDIIRLNKLEDTITLIKGKIEEVHLPVEKVDVIISEWMGYFLLFESMLDSVLYAKNKYLAKGGSVYPDICTISLVAVSDVNKHADRIAFWDDVYGFKMSCMKKAVIPEAVVEVLDPKTLISEPCGIKHIDCHTTSISDLEFSSDFTLKITRTSMCTAIAGYFDIYFEKNCHNRVVFSTGPQSTKTHWKQTVFLLEKPFSVKAGEALKGKVTVHKNKKDPRSLTVTLTLNNSTQTYGLQ
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Protein Length
Full Length of Isoform-1
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Molecular Weight
63 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PRMT3 (Protein Arginine Methyltransferase 3) is a member of the protein arginine methyltransferase family, which is involved in the post-translational modification of proteins through methylation of arginine residues. This modification plays a critical role in various biological processes, including gene expression regulation, signal transduction, and RNA processing. Research into PRMT3 has garnered interest due to its essential role in cellular function and its potential links to diseases such as cancer and neurodegenerative disorders. Specifically, PRMT3 is known to methylate several key proteins, including transcription factors and components of the spliceosome, thereby influencing cellular pathways. The study of recombinant PRMT3 protein provides insights into its catalytic activity, substrate specificity, and mechanisms of action. Understanding the structure and function of PRMT3 through recombinant protein technology can facilitate the development of targeted therapeutic strategies and help elucidate the contribution of arginine methylation to disease mechanisms. Furthermore, recombinant PRMT3 serves as a valuable tool for studying its biological roles and interactions in vitro, paving the way for future investigations into its implications in health and disease.
