Analytical Data
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基因名
Heparan Sulfate-6-O-Sulfotransferase-3
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简介
Heparan Sulfate-6-O-Sulfotransferase-3 (HS6ST3) is an enzyme involved in modifying heparan sulfate proteoglycan biosynthesis. It is predicted to be a membrane-bound integral component. HS6ST3 shows specific expression in the adult cortex (RPKM 1.3) and frontal lobe (RPKM 0.8), implying its potential role in determining the sulfation patterns of heparan sulfate in these contexts. Heparan Sulfate-6-O-Sulfotransferase-3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Heparan Sulfate-6-O-Sulfotransferase-3 protein, expressed by HEK293 , with N-His labeled tag.
- Application
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别名
Heparan-sulfate 6-O-sulfotransferase 3
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种属
Mouse
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表达系统
HEK293
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标签
N-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q9QYK4
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表达区间
P28-W470
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氨基酸序列
PSCTSSCTNFGEQLRSGEARPPAVPSPARRAQAPLDEWERRPQLPPPPRGPPEGSRGVAAPEDEDEDPGDPEEEEEEEEEEPDPEAPENGSLPRFVPRFNFTLKDLTRFVDFNIKGRDVIVFLHIQKTGGTTFGRHLVKNIRLEQPCSCKAGQKKCTCHRPGKKETWLFSRFSTGWSCGLHADWTELTNCVPAIMEKKDCPRNHSHTRNFYYITMLRDPVSRYLSEWKHVQRGATWKTSLHMCDGRSPTPDELPTCYPGDDWSGVSLREFMDCSYNLANNRQVRMLADLSLVGCYNLTFMNESERNTILLQSAKNNLKNMAFFGLTEFQRKTQFLFERTFNLKFISPFTQFNITRASNVDINDGARQHIEELNFLDMQLYEYAKDLFQQRYHHTKQLEHQRDRQKRREERRLQREHRAHRWPKEDRAMEGTVTEDYNSQVVRW
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蛋白长度
Lumenal Domain
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分子量
75-110 kDa.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Heparan sulfate (HS) is a glycosaminoglycan essential for various biological processes, including cell signaling, development, and tissue repair. It plays a crucial role in the interaction of growth factors, cytokines, and morphogens with their corresponding receptors, influencing cellular behavior in both normal physiology and disease states. Heparan sulfate-6-O-sulfotransferase-3 (HS6ST-3) is an important enzyme responsible for the addition of sulfate groups at the 6-position of glucosamine residues in HS chains, a modification that can significantly affect the biological activity and binding properties of HS. Dysregulation of HS sulfation patterns has been implicated in various pathological conditions, including cancer, where altered HS sulfation can enhance tumor progression and metastasis. Therefore, understanding the function and mechanism of HS6ST-3 is critical for elucidating its role in these processes. The production of recombinant HS6ST-3 protein enables detailed biochemical analysis, including substrate specificity, enzymatic activity, and structural studies. This research is crucial not only for understanding the fundamental biology of HS but also for developing potential therapeutic strategies targeting HS-related pathways in diseases. Additionally, recombinant HS6ST-3 can serve as a tool for the synthesis of specifically sulfated HS structures for use in drug development and regenerative medicine. By studying HS6ST-3, researchers aim to gain insights into the intricate regulation of heparan sulfate biosynthesis and its implications for health and disease.
