Analytical Data
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Gene name
3C protease
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简介
The 3C protease protein forms an icosahedral capsid with the VP2 and VP3 proteins, with a diameter of 300 angstroms. 3C protease Protein, Human rhinovirus 14 (GST) is the recombinant Virus-derived 3C protease protein, expressed by E. coli , with N-GST labeled tag.
- Application
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Alternative Names
Genome polyprotein
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Species
Virus
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Source
E. coli
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Tag
N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P03303
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Expression Region
G1538-Q1719
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Protein Length
Full Length of Protease 3C
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The 3C protease, derived from viral sources, is a cysteine protease that plays a crucial role in the maturation and replication of various picornaviruses, including enteroviruses and rhinoviruses. Its function involves cleaving viral polyproteins into functional structural and non-structural proteins, thereby facilitating the viral life cycle. Given its pivotal role, 3C protease has garnered significant interest for its potential applications in antiviral drug development and as a tool in protein engineering. The ability to study and manipulate this protease could lead to innovative strategies for inhibiting viral proliferation and creating novel therapeutic agents. Furthermore, recombinant 3C proteases can serve as valuable research tools in the field of molecular biology, particularly for the cleavage of fusion proteins in the production of recombinant proteins. By producing 3C protease in a recombinant system, researchers can explore its structural properties, enzymatic mechanisms, and interactions with other proteins, thereby advancing our understanding of viral pathogenesis and protein dynamics. Ongoing studies aim to optimize the production and purification of recombinant 3C protease, enhancing its utility in medical and biotechnological applications.
