Analytical Data
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Gene name
ACPS
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简介
ACPS Protein, Streptococcus pyogenes serotype M28 (sf9, His-Myc), a 4-phosphopantetheinyl transferase, activates two distinct acyl carrier proteins (ACPs) that are present in fatty acid synthase (FAS) systems FAS-I and FAS-II, the ACP-I domain and the mycobacterial ACP-II protein (ACPM), respectively[1].
- Application
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Alternative Names
acpS; M28_Spy1523Holo-[acyl-carrier-protein] synthase; Holo-ACP synthase; EC 2.7.8.7; 4'-phosphopantetheinyl transferase AcpS
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Species
Others
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Source
Baculovirus
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Tag
N-10*His;C-Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q48RM7
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Expression Region
M1-K118
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Protein Length
Full Length
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ACPS (Adenosine 5'-phosphosulfate kinase) is an essential enzyme involved in the sulfate activation pathway, which plays a critical role in the biosynthesis of sulfated biomolecules, including hormones, proteins, and glycosaminoglycans. The enzymatic activity of ACPS is crucial for cellular processes, as it catalyzes the phosphorylation of adenosine 5'-phosphosulfate (APS) to produce 3'-phosphoadenosine 5'-phosphosulfate (PAPS), the universal sulfate donor in biological systems. The study of recombinant ACPS has garnered interest due to its potential applications in biotechnology and medicine, particularly in understanding sulfation's role in diverse physiological and pathological processes. Moreover, insights gained from the structural and functional characterization of recombinant ACPS can enhance our understanding of enzyme mechanisms and allow for the development of inhibitors that could be useful in modulating sulfation-related pathways. This research is significant in the context of drug development and disease treatment, particularly in conditions where sulfation processes are altered, such as cancer and metabolic disorders. By producing and studying the recombinant form of ACPS, researchers aim to elucidate its activity, regulation, and potential as a target for therapeutic intervention.
