Analytical Data
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Gene name
GPT
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简介
GPT proteins play a key role in cellular nitrogen metabolism by catalyzing the reversible ammonia interaction between alanine and 2-oxoglutarate to produce pyruvate and glutamate products. This enzyme activity is an integral part of the dynamic balance of amino acid metabolism and contributes to the interconversion of key metabolites. GPT Protein, Rat (sf9, His) is the recombinant rat-derived GPT protein, expressed by Sf9 insect cells , with C-His labeled tag.
- Application
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Alternative Names
Alanine aminotransferase 1; ALT1; GPT 1; Aat1
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Species
Rat
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Source
Baculovirus
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Tag
C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P25409
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Expression Region
M1-S496
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Protein Length
Full Length
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Molecular Weight
48 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Research on GPT (glutamate pyruvate transaminase) recombinant proteins has gained significant attention due to their crucial role in metabolic pathways and potential therapeutic applications. GPT, an enzyme primarily found in the liver and kidneys, catalyzes the reversible transfer of amino groups between glutamate and pyruvate, leading to the production of α-ketoglutarate and alanine. This reaction is vital for amino acid metabolism and energy homeostasis. The recombinant production of GPT proteins allows for detailed biochemical studies, facilitating the exploration of their structure-function relationships and enzymatic mechanisms. Moreover, the ability to produce these proteins in a controlled manner enables researchers to investigate their roles in various diseases, particularly metabolic disorders and liver dysfunctions. As the understanding of GPT's involvement in pathological conditions grows, there is increasing interest in leveraging recombinant GPT proteins for diagnostic and therapeutic strategies. This includes the potential development of novel drugs targeting metabolic pathways influenced by GPT activity, as well as the use of GPT as a biomarker for assessing liver function. The advancement of genetic engineering and protein expression systems has further propelled research into GPT recombinant proteins, making them invaluable tools in both basic and applied biomedical research.
