Analytical Data
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Gene name
CHST11
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简介
CHST11 Protein catalyzes sulfate transfer to position 4 of the GalNAc residue in chondroitin, the main proteoglycan in cartilage and cell surfaces. It can sulfate Gal residues in desulfated dermatan sulfate, with a preference for GlcA->GalNAc units over IdoA->GalNAc units, and does not form 4,6-di-O-sulfated GalNAc when chondroitin sulfate C is the acceptor. CHST11 Protein, Human (sf9, His) is the recombinant human-derived CHST11 protein, expressed by Sf9 insect cells , with C-His labeled tag.
- Application
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Alternative Names
Carbohydrate sulfotransferase 11; Chondroitin 4-sulfotransferase 1; C4ST1
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Species
Human
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Source
Baculovirus
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Tag
C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NPF2-2
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Expression Region
M36-E347
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Protein Length
Partial
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Molecular Weight
43 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CHST11, or carbohydrate sulfotransferase 11, is an enzyme that plays a crucial role in the modification of glycosaminoglycans (GAGs) by adding sulfate groups, which are essential for various biological processes including cell signaling, adhesion, and overall tissue function. The study of CHST11 recombinant protein is significant in understanding its function in pathological conditions such as cancer metastasis, cardiovascular diseases, and developmental disorders. Its sulfation activity influences the structure and function of GAGs, ultimately affecting cellular mechanisms and interactions. Previous research has identified CHST11 as a potential biomarker for certain diseases, making it a target for therapeutic interventions. The production of recombinant CHST11 protein allows for in-depth biochemical studies, enabling researchers to elucidate the enzyme's structural properties, substrate specificity, and regulatory mechanisms. Furthermore, exploring its functional implications in various biological contexts can provide insights into the development of novel treatments and diagnostic tools. The advent of recombinant DNA technology has facilitated the expression and purification of CHST11, thus advancing our understanding of its biological significance and potential applications in medicine and biotechnology.
