Analytical Data
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Gene name
STAT3
- Application
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Alternative Names
STAT3; signal transducer and activator of transcription 3 (acute-phase response factor); signal transducer and activator of transcription 3; APRF
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Species
Human
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Source
E. coli
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Tag
N-terminal His-Tag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P40763
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Expression Region
127-688aa
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AA Sequence
GQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLK SQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKT LTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQ QKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTG VQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNM EESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGL KIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQV AEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKG FSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSE SSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNIL VSPLVYLYPDIPKEEAFGKYCR
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Molecular Weight
67.7kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
Signal Transducer and Activator of Transcription 3 (STAT3) is a critical transcription factor involved in various cellular processes, including proliferation, survival, and differentiation. Dysregulation of STAT3 activity has been implicated in numerous diseases, particularly in cancers, where its persistent activation contributes to tumorigenesis and the survival of malignant cells. Research on recombinant STAT3 proteins has gained momentum due to their potential therapeutic applications and role in elucidating the pathways related to cell signaling and cancer progression. Scientists have employed various expression systems, such as bacterial, yeast, and mammalian cells, to produce functional STAT3 proteins, enabling the study of its structure, dynamics, and interactions with other cellular molecules. Understanding the mechanisms by which STAT3 is activated and functions can provide insights into novel drug targets and therapeutic strategies. Furthermore, the development of STAT3 inhibitors is of considerable interest in cancer treatment, as they may offer a way to impede aberrant signaling pathways. Overall, the study of recombinant STAT3 proteins is essential for advancing our knowledge of its biological significance and exploring new avenues for clinical intervention.
