Analytical Data
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基因名
NRG1-beta 1
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简介
HRG1-β1 belongs to a family of structurally-related polypeptide growth factors derived from alternatively spliced genes, induces Fn14 expression in MCF7 cells. NRG1-beta 1 Protein, Human (245a.a, HEK293, Fc) is the recombinant human-derived NRG1-beta 1 protein, expressed by HEK293 , with N-hFc labeled tag.
- Application
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生物活性
1. Measured by its binding ability in a functional ELISA.Immobilized Human ERBB3 His at 2 μg/mL (100 μl/well) can bind Human NRG1 Fc , the EC50 of Human NRG1 Fc is 8-40 ng/mL. 2. Measured by its binding ability in a functional ELISA.Immobilized Rhesus ERBB3 His at 2 μg/mL (100 μl/well) can bind Human NRG1 Fc , the EC50 of Human NRG1 Fc is 20-80 ng/mL. 3. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 this effect is 0.1087-7.029 ng/mL, corresponding to a specific activity is 1.4227×105 - 9.20×106 units/mg. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 this effect is 7.029 ng/mL, corresponding to a specific activity is 1.4227×105 units/mg.
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别名
Pro-neuregulin-1; Neuregulin-1; ARIA; HRG; NRG1; GGF; HGL; HRGA; NDF; SMDF
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种属
Human
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表达系统
HEK293
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标签
N-hFc
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纯度
Greater than 95% as determined by reducing SDS-PAGE.
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蛋白编号
Q02297-6
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表达区间
S2-K246
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氨基酸序列
SERKEGRGKGKGKKKERGSGKKPESAAGSQSPALPPRLKEMKSQESAAGSKLVLRCETSSEYSSLRFKWFKNGNELNRKNKPQNIKIQKKPGKSELRINKASLADSGEYMCKVISKLGNDSASANITIVESNEIITGMPASTEGAYVSSESPIRISVSTEGANTSSSTSTSTTGTSHLVKCAEKEKTFCVNGGECFMVKDLSNPSRYLCKCPNEFTGDRCQNYVMASFYKHLGIEFMEAEELYQK
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蛋白长度
Partial
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分子量
58-83 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NRG1-beta 1, or Neuregulin 1 beta 1, is a vital member of the neuregulin family of proteins, which play crucial roles in various physiological processes, including neuronal development, cardiac function, and immune response. Research into NRG1-beta 1 has gained significant momentum due to its involvement in crucial signaling pathways that influence cell growth, differentiation, and survival. Dysregulation of NRG1-beta 1 has been implicated in several neurodevelopmental disorders, such as schizophrenia, and cardiac conditions, highlighting its therapeutic potential. Additionally, NRG1-beta 1 has shown promise in promoting neuroprotection and repair following injury, making it a compelling target for regenerative medicine. The recombinant expression of NRG1-beta 1 enables detailed studies into its biological functions and facilitates the exploration of its applications in drug development and biotechnology. Understanding the mechanisms of NRG1-beta 1 signaling could provide insights into its therapeutic implications, paving the way for novel treatment strategies targeting a variety of diseases linked to its altered expression. As research progresses, the elucidation of the structural and functional properties of NRG1-beta 1 will enhance our knowledge and allow for the design of targeted therapies, contributing to advancements in the fields of neurology, cardiology, and beyond.
