Analytical Data
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基因名
NEK3
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简介
NEK3 Protein influences neuronal morphogenesis and polarity through effects on microtubules. NEK3 Protein regulates microtubule acetylation in neurons. NEK3 Protein contributes to prolactin-mediated phosphorylation of PXN and VAV2. NEK3 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived NEK3 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Application
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别名
Serine/threonine-protein kinase Nek3; HSPK 36; NimA-related protein kinase 3
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种属
Mouse
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表达系统
Baculovirus
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标签
N-His;N-GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
ABK42288
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表达区间
M1-A509
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蛋白长度
Full Length
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分子量
72 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NEK3 (NIMA-related kinase 3) is a serine/threonine kinase that belongs to the NEK family, which is crucial for cell cycle regulation and mitotic progress. Recent studies have indicated that NEK3 plays a significant role in various cellular processes, including cilia formation, cell division, and response to DNA damage, making it a subject of growing interest in cancer and other diseases. The reorganization of NEK3 within tumor cells has been associated with altered cell proliferation and survival, highlighting its potential as a biomarker for cancer prognosis. Additionally, NEK3 has been implicated in the regulation of ciliary dynamics, suggesting its involvement in developmental disorders related to cilia dysfunction. Researchers are investigating the structural and functional characteristics of recombinant NEK3 protein to elucidate its exact role in signaling pathways and its interactions with other cellular components. By employing techniques such as protein purification and characterization, studies aim to develop NEK3-targeted therapies that could mitigate its aberrant activity in disease states, thereby advancing our understanding of its biological functions and therapeutic potential. Overall, the investigation of NEK3 recombinant protein presents a promising avenue for uncovering novel insights into its mechanisms and applications in health and disease.
