Analytical Data
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Gene name
VRK1
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简介
The VRK1 protein is a serine/threonine kinase that complexly regulates the cell cycle, nuclear condensation, and transcription. It critically induces Golgi disassembly during the cell cycle via PLK3 phosphorylation, thereby affecting Golgi fragmentation. VRK1 Protein, Human (sf9) is the recombinant human-derived VRK1 protein, expressed by Sf9 insect cells, with tag free.
- Application
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Alternative Names
Serine/threonine-protein kinase VRK1; Vaccinia-related kinase 1; VRK1
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Species
Human
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Source
Baculovirus
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q99986
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Expression Region
Q99986 (N-G&P, M1-K396)
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Protein Length
Full Length
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Molecular Weight
47 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
VRK1 (vaccinia-related kinase 1) is a serine/threonine kinase that plays a critical role in cell proliferation, differentiation, and stress response. Its significance in cellular processes has drawn attention due to its involvement in various biological functions, particularly in the regulation of the cell cycle and response to DNA damage. Studies have demonstrated that VRK1 can phosphorylate key substrates, influencing signaling pathways that are crucial for cellular homeostasis. Moreover, aberrant expression or mutation of VRK1 has been implicated in several diseases, including cancer, where it may contribute to uncontrolled proliferation and progression of tumors. Research into VRK1 recombinant protein aims to unravel its functional mechanisms and potential as a therapeutic target. By producing VRK1 in a recombinant form, scientists can investigate its enzymatic activity, substrate specificity, and interaction with other proteins in a controlled environment. This research is essential for elucidating the kinase's role in pathophysiological conditions and could lead to novel strategies for disease intervention, highlighting the importance of VRK1 in both basic biology and clinical applications.
