Analytical Data
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Gene name
RIOK1
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简介
The RIOK1 protein is critical in the final cytoplasmic maturation of the 40S ribosomal subunit, actively processing 18S-E pre-rRNA into mature 18S rRNA. RIOK1 is critical for NOB1 and PNO1 recycling of 40S late precursors, and its association with 40S late subunits suggests involvement in a translation-like checkpoint cycle. RIOK1 Protein, Human (sf9, His-GST) is the recombinant human-derived RIOK1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Application
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Alternative Names
Serine/threonine-protein kinase RIO1; RIO kinase 1; RIO1
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Species
Human
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Source
Baculovirus
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Tag
N-His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BRS2
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Expression Region
M1-K568
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Protein Length
Full Length
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Molecular Weight
94 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RIOK1, a member of the RIO (Ribonucleotide reductase and tRNA-guanine transglycosylase) family, has garnered significant interest in recent years due to its essential role in ribosome biogenesis and cellular metabolism. As a cytoplasmic protein, RIOK1 is involved in the maturation of ribosomal RNA and the assembly of ribosomal subunits, processes critical for protein synthesis in eukaryotic cells. Studies have indicated that RIOK1 is not only crucial for ribosome assembly but also plays a role in cell proliferation and survival, highlighting its potential implications in various diseases, including cancer. Abnormal expression of RIOK1 has been observed in several tumor types, suggesting that it could serve as a novel biomarker or therapeutic target. The investigation of RIOK1 as a recombinant protein has opened new avenues for understanding its structure-function relationships and interactions with other cellular components. By producing RIOK1 in a controlled laboratory setting, researchers aim to elucidate its biochemical properties and functional mechanisms, which could ultimately contribute to the development of targeted therapies that modulate ribosomal activity in disease states. Overall, the study of RIOK1 is poised to enhance our understanding of fundamental cellular processes and their alterations in disease, paving the way for innovative therapeutic strategies.
