Analytical Data
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Gene name
PHKG1
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简介
PHKG1 is the catalytic subunit of phosphorylase b kinase (PHK), which plays a key role in the neural and hormonal regulation of glycogenolysis (glycogen breakdown) by phosphorylating and activating glycogen phosphorylase. PHKG1 Protein, Human (Active, sf9, GST) is the recombinant human-derived PHKG1, expressed by Sf9 insect cells, with GST labeled tag.
- Application
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Species
Human
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Source
Baculovirus
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Tag
GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q16816-1
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Expression Region
T2-Y387
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PHKG1 (Phosphorylase Kinase Gamma 1) is a crucial enzyme that plays a significant role in glycogen metabolism, primarily by regulating glycogen phosphorylase through phosphorylation. This enzyme is essential for energy mobilization during muscular contraction and other physiological processes. Mutations or dysregulation of PHKG1 have been implicated in various metabolic disorders, including glycogen storage diseases, which can lead to severe muscle weakness and exercise intolerance. The study of PHKG1 recombinant protein is vital to understanding its structure-function relationships and regulatory mechanisms. By producing and characterizing recombinant PHKG1, researchers can investigate its enzymatic activity, substrate specificity, and interactions with other proteins. Additionally, studying the recombinant protein provides insights into the pathological mechanisms underlying PHKG1-related diseases and paves the way for potential therapeutic interventions. Understanding PHKG1's role in cellular metabolism may also contribute to the development of strategies to enhance muscle performance and treat metabolic disorders. Thus, the research on PHKG1 recombinant protein is essential for both basic science and clinical applications, offering a foundation for future studies aiming to unravel the complexities of glycogen metabolism and its associated diseases.
