Analytical Data
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Gene name
CDK1-CCNE2
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简介
The CDK1/CDC2-cyclin-B complex is a key regulator in the cell cycle. It plays an important role in cell division and development and regulates a variety of cellular activities, including chromosome segregation, nuclear membrane rupture, cytokinesis, etc. The activity of CDK1 is regulated by a variety of phosphorylation and dephosphorylation, thereby controlling the progression of the cell cycle and the DNA repair process. CDK1-CCNE2 Protein, Human (sf9, GST, His, Flag) is the recombinant human-derived CDK1-CCNE2, expressed by Sf9 insect cells, with N-His, N-GST, N-Flag labeled tag.
- Application
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Species
Human
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Source
Baculovirus
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Tag
N-His;N-GST;N-Flag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P06493-1 (M1-M297)&O96020-1
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Expression Region
P06493-1 (M1-M297)&O96020-1 (S2-H404 )
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CDK1, or Cyclin-Dependent Kinase 1, is a crucial regulator of cell cycle progression, particularly in the transition from the G2 phase to mitosis. It functions in complex with cyclins, with CCNE2 (Cyclin E2) being one such partner that plays a significant role in linking cell cycle regulation to other cellular processes. The CDK1-CCNE2 complex is thought to be involved in the precise control of cell division, DNA replication, and responses to DNA damage. Dysregulation of this complex has been implicated in various cancers, making it a target of interest for therapeutic interventions. Research on CDK1-CCNE2 recombinant protein has focused on its structural and functional characterization, aiming to understand the molecular mechanisms underlying its activity and regulation. By utilizing recombinant DNA technology, scientists can produce this complex in vitro, allowing for detailed studies of its enzymatic activity, binding interactions, and potential as a biomarker or drug target. Understanding the biochemistry and dynamics of the CDK1-CCNE2 complex is essential for developing strategies to combat cancer and other diseases associated with cell cycle dysregulation.
