Analytical Data
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基因名
Histone H3
- Application
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别名
H3/n; H3/o; HIST2H3D; HIST2H3C
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种属
Mouse
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P84228
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表达区间
Met1~Ala136
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分子量
18kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Histone H3 recombinant protein has garnered significant interest in the field of molecular biology due to its critical role in chromatin structure and gene regulation. Histones, particularly H3, are fundamental components of nucleosomes, which package DNA into a compact, organized form within the nucleus of eukaryotic cells. The post-translational modifications of histone H3, such as methylation, acetylation, and phosphorylation, are known to influence gene expression patterns and are closely linked to various cellular processes, including replication, repair, and transcription. The study of recombinant Histone H3 allows researchers to explore these modifications in a controlled environment, facilitating their understanding of epigenetic mechanisms. Furthermore, aberrations in histone modifications have been associated with several diseases, including cancer, making the investigation of H3 variants and their interactions crucial for therapeutic development. By utilizing recombinant technology, scientists can produce specific histone variants for in vitro studies, enabling them to dissect the influence of particular histone modifications on chromatin dynamics and gene activity. Ultimately, this research contributes to a deeper understanding of the epigenetic landscape and its implications in health and disease.
