Analytical Data
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Gene name
Lipocalin-2/NGAL
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简介
Lipocalin-2/NGAL is a multifaceted iron transporter involved in multiple biological processes, including apoptosis, innate immunity, and kidney development. Lipocalin-2 dynamically affects cellular iron concentration through binding to the siderophore 2,3-dihydroxybenzoate. Lipocalin-2/NGAL Protein, Rat (sf9, His) is the recombinant rat-derived Lipocalin-2/NGAL protein, expressed by Sf9 insect cells , with C-His labeled tag.
- Application
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Alternative Names
Neutrophil gelatinase-associated lipocalin; NGAL; Lipocalin 24p3; p25; LCN2
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Species
Rat
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Source
Baculovirus
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Tag
C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P30152
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Expression Region
Q21-N198
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Protein Length
Full Length of Mature Protein
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Molecular Weight
25 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), is a 25-kDa protein belonging to the lipocalin family, primarily involved in the innate immune response. It is secreted by various cell types, including neutrophils, epithelial cells, and adipocytes, and plays a crucial role in iron metabolism and inflammation. Elevated levels of Lipocalin-2/NGAL have been associated with several pathological conditions, such as acute kidney injury, chronic kidney disease, obesity, and certain cancers, making it a critical biomarker for these diseases. Research on recombinant Lipocalin-2/NGAL has gained traction due to its potential therapeutic applications. Recombinant protein production enables detailed studies of its biological functions, including its role in modulating immune responses, cell survival, and apoptosis. By understanding the molecular mechanisms underlying Lipocalin-2/NGAL’s actions, researchers aim to develop novel diagnostic tools and therapeutic interventions targeting various diseases. Moreover, investigations into the protein's structural characteristics and interactions with other biomolecules provide insights into its functional specificity and potential for clinical use. Overall, the study of recombinant Lipocalin-2/NGAL emphasizes its significance in biomedical research, highlighting its dual role as both a biomarker and a therapeutic target, thereby opening new avenues for the management of inflammation-related diseases and improving patient outcomes.
